Rabbit anti-Human, Mouse PYM1 Polyclonal Antibody | anti-PYM1 antibody

PYM1 Antibody

Cat. Num. AAA9630297

• Gene Names: WIBG; PYM
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PYM1; Polyclonal Antibody; PYM1 Antibody; A030010B05Rik; MGC13064; Partner of Y14 and mago; Protein wibg homolog; PYM; PYM homolog 1 exon junction complex-associated factor; pym1; PYM1_HUMAN; WIBG; Within bgcn homolog; anti-PYM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PYM1 Antibody detects endogenous levels of PYM1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PYM1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PYM1.
• Conjugation: Unconjugated
• Similarity: The eIF2A-like region shares sequence similarity with eIF2A and mediates the interaction with the 40S ribosomal subunit and the 48S preinitiation complex. Belongs to the pym family.
• Subunit Structure: Interacts (via N-terminus) with MAGOH and RBM8A; the interaction is direct. Associates (eIF2A-like region) with the 40S ribosomal subunit and the 48S preinitiation complex.
• Function: Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by whiledid not detect RNA-binding activity independently of the EJC.
• Note: huttles between the nucleus and the cytoplasm (PubMed:14968132). Nuclear export is mediated by XPO1/CRM1 (PubMed:14968132).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PYM1 antibody

Cytoplasm; Nucleus; Nucleoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 219803946
• NCBI GeneID: 84305
• NCBI Accession #: NP_001137325.1
• NCBI GenBank Nucleotide #: NM_001143853.1
• UniProt Accession #: Q9BRP8; Q8IXJ8; Q8N8E7; B6ZDM5
• Molecular Weight: 22,705 Da
• NCBI Official Full Name: partner of Y14 and mago isoform 2
• NCBI Official Synonym Full Names: within bgcn homolog (Drosophila)
• NCBI Official Symbol: WIBG
• NCBI Official Synonym Symbols: PYM
• NCBI Protein Information: partner of Y14 and mago; protein wibg homolog
• UniProt Protein Name: Partner of Y14 and mago
• UniProt Gene Name: WIBG
• UniProt Synonym Gene Names: PYM
• UniProt Entry Name: WIBG_HUMAN

Uniprot Description

WIBG: a conserved protein of unknown function. A potential RNA-binding protein.

Protein type: RNA-binding; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus

Molecular Function: protein binding; ribosome binding

Biological Process: positive regulation of translation; mRNA catabolic process, nonsense-mediated decay