Rabbit anti-Human PYGO1 Polyclonal Antibody | anti-PYGO1 antibody

PYGO1, CT (PYGO1, Pygopus homolog 1) (PE)

Cat. Num. AAA6339381

• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PYGO1; Polyclonal Antibody; CT (PYGO1; Pygopus homolog 1) (PE); Pygopus homolog 1; anti-PYGO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PYGO1 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PYGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from the C-terminal region of human PYGO1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PYGO1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PYGO1 antibody detected the PYGO1 protein (arrow).)

Immunohistochemistry (IHC)

(PYGO1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PYGO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PYGO1 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PYGO1 antibody

PYGO1 is involved in signal transduction through the Wnt pathway.

Product Categories/Family for anti-PYGO1 antibody

Antibodies; Signal Transduction

References

Frank, B., et al. Carcinogenesis (2010). In press: Fiedler, M., et al. Mol. Cell 30(4):507-518(2008). Townsley, F.M., et al. J. Biol. Chem. 279(7):5177-5183(2004). Thompson, B., et al. Nat. Cell Biol. 4(5):367-373(2002). Kramps, T., et al. Cell 109(1):47-60(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 30911103
• NCBI GeneID: 26108
• NCBI Accession #: NP_056432.1
• NCBI GenBank Nucleotide #: NM_015617.2
• UniProt Accession #: Q9Y3Y4; A7Y2D6
• Molecular Weight: 45,318 Da
• NCBI Official Full Name: pygopus homolog 1
• NCBI Official Synonym Full Names: pygopus family PHD finger 1
• NCBI Official Symbol: PYGO1
• NCBI Protein Information: pygopus homolog 1; pygopus-like protein 1
• UniProt Protein Name: Pygopus homolog 1
• Protein Family: Pygopus
• UniProt Gene Name: PYGO1
• UniProt Entry Name: PYGO1_HUMAN

Uniprot Description

Function: Involved in signal transduction through the Wnt pathway.

Subunit structure: Binds to BCL9 via The PHD-type zinc finger motiv, and thereby becomes part of the nuclear beta-catenin/TCF complex. Found in a complex with BCL9L, CDC73, CTNNB1 and PYGO1.

Subcellular location: Nucleus

Probable.

Sequence similarities: Contains 1 PHD-type zinc finger.