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Western Blot (WB) (Western blot analysis of Rat Liver showing detection of ~78 kDa MMP9 protein using Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa. Other Band(s): 64 kDa.)

Rabbit anti-Rat MMP9 Polyclonal Antibody | anti-MMP9 antibody

MMP9 Antibody: Biotin

Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Rat
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
MMP9; Polyclonal Antibody; MMP9 Antibody: Biotin; Rabbit Anti-Human MMP9 Polyclonal; MMP 9 Antibody; CLG4B Antibody; Matrix metallopeptidase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase) Antibody; Matrix Metalloproteinase 9 Antibody; Macrophage gelatinase Antibody; MMP9_HUMAN Antibody; EC:3.4.24.35 Antibody; MMP-9 Antibody; Gelatinase 92 KD Antibody; GELB Antibody; CLG 4B Antibody; Type V collagenase Antibody; 92 kDa gelatinase Antibody; Gelatinase B Antibody; 82 kDa matrix metalloproteinase-9 Antibody; Collagenase type IV 92 KD Antibody; MANDP2 Antibody; Matrix metalloproteinase-9 Antibody; Gelatinase beta Antibody; Collagenase Type 4 beta Antibody; GelatinaseB Antibody; EC 3.4.24.35 Antibody; 92 kDa type IV collagenase Antibody; MMP9 Antibody; anti-MMP9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
Detects 78 kDa, 64 kDa. Actual molecular weight is 78 kDa. 64 kDa is a known active form of the protein.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
707
Applicable Applications for anti-MMP9 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
MMP9
Immunogen
Synthetic peptide from the mid-protein of Human MMP9 (aa. 400-500)
Immunogen Species
Human
Cellular Localization
Secreted; Extracellular Space; Extracellular Matrix
Conjugate
Biotin
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Produced by normal alveolar macrophages and granulocytes.
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat Liver showing detection of ~78 kDa MMP9 protein using Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa. Other Band(s): 64 kDa.)

Western Blot (WB) (Western blot analysis of Rat Liver showing detection of ~78 kDa MMP9 protein using Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MMP9 Polyclonal Antibody (SPC-756) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa. Other Band(s): 64 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78,458 Da
NCBI Official Full Name
matrix metalloproteinase-9 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
MMP9
NCBI Official Synonym Symbols
GELB; CLG4B; MMP-9; MANDP2
NCBI Protein Information
matrix metalloproteinase-9
UniProt Protein Name
Matrix metalloproteinase-9
Protein Family
UniProt Gene Name
MMP9
UniProt Synonym Gene Names
CLG4B; MMP-9; GELB

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Research Articles on MMP9

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Product Notes

The MMP9 mmp9 (Catalog #AAA8001050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP9 Antibody: Biotin reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the MMP9 mmp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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