Rabbit PYGM Polyclonal Antibody | anti-PYGM antibody

Rabbit Polyclonal to Human PYGM

Cat. Num. AAA240010

• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PYGM; Polyclonal Antibody; Rabbit Polyclonal to Human PYGM; Anti-PYGM Antibody (C-Terminus) IHC-plus; GPMM; Myophosphorylase; Phosphorylase; glycogen muscle; Human PYGM; anti-PYGM antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PYGM / Phosphorylase b. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RARRES1 (57%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 842

• Applicable Applications for anti-PYGM antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (20 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 14 amino acid peptide from C-terminus of human PYGM / Phosphorylase b. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey (100%); Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Pig, Armadillo (93%).
• Immunogen Type: Synthetic phospho-peptide
• Immunogen: PYGM antibody was raised against synthetic 14 amino acid peptide from C-terminus of human PYGM / Phosphorylase b. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey (100%); Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Pig, Armadillo (93%); Dog, Panda, Horse (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-PYGM / Phosphorylase b antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

NCBI and Uniprot Product Information

• NCBI GI #: 5032009
• NCBI GeneID: 5837
• NCBI Accession #: NP_005600.1
• NCBI GenBank Nucleotide #: NM_005609.2
• UniProt Accession #: P11217; A0AVK1; A6NDY6
• Molecular Weight: 87,317 Da
• NCBI Official Full Name: glycogen phosphorylase, muscle form isoform 1
• NCBI Official Synonym Full Names: phosphorylase, glycogen, muscle
• NCBI Official Symbol: PYGM
• NCBI Protein Information: glycogen phosphorylase, muscle form; myophosphorylase
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGM
• UniProt Entry Name: PYGM_HUMAN

NCBI Description

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Phosphorylase; Transferase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytosol

Molecular Function: glycogen phosphorylase activity; nucleotide binding; pyridoxal phosphate binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Glycogen Storage Disease V