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Western Blot (WB) (Western blot analysis of anti-PXK Pab (MBS9211142) in Hela cell line lysate (35ug/lane). PXK(arrow) was detected using the purified Pab.)

Rabbit PXK Polyclonal Antibody | anti-PXK antibody

PXK Antibody (Center)

Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PXK; Polyclonal Antibody; PXK Antibody (Center); PX domain-containing protein kinase-like protein; Modulator of Na;K-ATPase; MONaKA; PXK;; anti-PXK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-251 amino acids from the Central region of human PXK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
221-251
Sequence Length
377
Applicable Applications for anti-PXK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-PXK Pab (MBS9211142) in Hela cell line lysate (35ug/lane). PXK(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-PXK Pab (MBS9211142) in Hela cell line lysate (35ug/lane). PXK(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PXK (arrow) using rabbit polyclonal PXK Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PXK gene.)

Western Blot (WB) (Western blot analysis of PXK (arrow) using rabbit polyclonal PXK Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PXK gene.)
Related Product Information for anti-PXK antibody
PXK is widely expressed in all tissues examined except in heart. It binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. However, the protein kinase domain is predicted to be catalytically inactive.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
43849
NCBI Official Full Name
PXK protein
UniProt Protein Name
PXK protein
UniProt Gene Name
PXK
UniProt Entry Name
Q32NE9_HUMAN

Uniprot Description

Slob: Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity. Belongs to the protein kinase superfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; Slob family

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; nucleotide binding; actin binding; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: regulation of synaptic transmission; inflammatory response; negative regulation of ion transport; negative regulation of ATPase activity; protein amino acid phosphorylation

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Product Notes

The PXK pxk (Catalog #AAA9211142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 221-251. The PXK Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PXK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PXK pxk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PXK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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