Rabbit anti-Human PXK Polyclonal Antibody | anti-PXK antibody

PXK, ID (PX Domain-containing Protein Kinase-like Protein, Modulator of Na,K-ATPase, MONaKA) (Biotin)

Cat. Num. AAA6496157

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PXK; Polyclonal Antibody; ID (PX Domain-containing Protein Kinase-like Protein; Modulator of Na; K-ATPase; MONaKA) (Biotin); anti-PXK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PXK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 377

• Applicable Applications for anti-PXK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center-terminal region of human PXK (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PXK Pab (AP7017c) in Hela cell line lysate (35ug/lane). PXK(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PXK (arrow) using rabbit polyclonal PXK Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PXK gene.)

Product Categories/Family for anti-PXK antibody

Antibodies; Enzymes; ATPase

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 80477235
• UniProt Accession #: Q32NE9
• Molecular Weight: 43.849
• NCBI Official Full Name: PXK protein, partial
• UniProt Protein Name: PXK protein
• Protein Family: PX domain-containing protein kinase-like protein
• UniProt Gene Name: PXK
• UniProt Entry Name: Q32NE9_HUMAN

Uniprot Description

Slob: Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity. Belongs to the protein kinase superfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; Slob family

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; nucleotide binding; actin binding; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: regulation of synaptic transmission; inflammatory response; negative regulation of ion transport; negative regulation of ATPase activity; protein amino acid phosphorylation