Rabbit anti-Human, Mouse PUMA Polyclonal Antibody | anti-Bbc3 antibody

PUMA (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1)

Cat. Num. AAA610635

• Gene Names: Bbc3; Puma
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: PUMA; Polyclonal Antibody; PUMA (p53 Upregulated Modulator of Apoptosis; BCL2 Binding Component 3; BCL-2 Binding Component 3; BBC3; JFY1; JFY-1; p53 Up-regulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1); Anti -PUMA (p53 Upregulated Modulator of Apoptosis; anti-Bbc3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PUMA a in WB and immunohistochemistry applications (Product citation 1). It is supplied at 0.5 mg/ml. Species Crossreactivity: Human, Mouse.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-Bbc3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: This antibody was generated by immunizing rabbit with synthetic peptides of human PUMAa (CT).
• Positive Control: Jurkat or NIH 3T3 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Bbc3 antibody

A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Product Categories/Family for anti-Bbc3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 27542559
• NCBI GeneID: 317673
• UniProt Accession #: Q80ZG6
• Molecular Weight: 20,718 Da
• NCBI Official Full Name: PUMA
• NCBI Official Synonym Full Names: Bcl-2 binding component 3
• NCBI Official Symbol: Bbc3
• NCBI Official Synonym Symbols: Puma
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: Bbc3
• UniProt Synonym Gene Names: Puma
• UniProt Entry Name: BBC3_RAT

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; glial cell differentiation; positive regulation of protein homooligomerization; positive regulation of apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; brain development; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; negative regulation of growth