Western Blot (WB)
(Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human PUM2 Polyclonal Antibody | anti-PUM2 antibody
PUM2 (S182) (Pumilio 2, Pumilio Homolog 2, Pumilio-2, KIAA0235, PUMH2) (APC)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PUM2 (arrow) using PUM2 Antibody (S182). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
Pumilio-2: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Motility/polarity/chemotaxis; Translation
Chromosomal Location of Human Ortholog: 2p24.1
Cellular Component: nuclear membrane; stress granule; perinuclear region of cytoplasm; cytoplasm
Molecular Function: protein binding
Biological Process: regulation of translation; stress granule assembly
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Product Notes
The PUM2 pum2 (Catalog #AAA6496123) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PUM2 (S182) (Pumilio 2, Pumilio Homolog 2, Pumilio-2, KIAA0235, PUMH2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PUM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA, Immunohistochemistry (IHC). WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PUM2 pum2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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