Rabbit anti-Human PUF60 Polyclonal Antibody | anti-PUF60 antibody

PUF60 Antibody, HRP conjugated

Cat. Num. AAA7051377

• Gene Names: PUF60; FIR; VRJS; RoBPI; SIAHBP1
• Reactivity: Human
• Applications: ELISA
• Purity: >95%, Protein G purified
• Synonyms: PUF60; Polyclonal Antibody; PUF60 Antibody; HRP conjugated; Poly(U)-binding-splicing factor PUF60; 60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-bind; FIR; ROBPI; SIAHBP1; anti-PUF60 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 516

• Applicable Applications for anti-PUF60 antibody: ELISA (EIA)
• Immunogen: Recombinant Human Poly(U)-binding-splicing factor PUF60 protein (302-413AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: HRP
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-11097 / sc-133583
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Related Product Information for anti-PUF60 antibody

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 209862867
• NCBI GeneID: 22827
• NCBI Accession #: NP_001129505.1
• NCBI GenBank Nucleotide #: NM_001136033.2
• UniProt Accession #: Q9UHX1; Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8
• Molecular Weight: 55,399 Da
• NCBI Official Full Name: poly(U)-binding-splicing factor PUF60 isoform c
• NCBI Official Synonym Full Names: poly(U) binding splicing factor 60
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; VRJS; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60
• UniProt Protein Name: Poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor
• UniProt Gene Name: PUF60
• UniProt Synonym Gene Names: FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
• UniProt Entry Name: PUF60_HUMAN

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cell-cell adherens junction; cyclin-dependent protein kinase activating kinase holoenzyme complex; nucleoplasm

Molecular Function: identical protein binding; protein binding

Disease: Verheij Syndrome