Rabbit PTPN6 Polyclonal Antibody | anti-PTPN6 antibody

PTPN6, Phosphorylated (Y536) (Protein Tyrosine Phosphatase Non-receptor Type 6, HCP, HCPH, SHP1, SHP-1, HPTP1C, PTP-1C, SHP-1L, SH-PTP1) (MaxLight 490)

Cat. Num. AAA6434246

• Gene Names: PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PTPN6; Polyclonal Antibody; Phosphorylated (Y536) (Protein Tyrosine Phosphatase Non-receptor Type 6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1) (MaxLight 490); anti-PTPN6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN6, phosphorylated Y536. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-PTPN6 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Phospho-specific peptide corresponding to residues surrounding Y536 of human PTPN6 (P29350).
• Conjugate: MaxLight490
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 212924.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using 212924.)

Related Product Information for anti-PTPN6 antibody

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Product Categories/Family for anti-PTPN6 antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 18104989
• NCBI GeneID: 5777
• NCBI Accession #: NP_002822.2
• NCBI GenBank Nucleotide #: NM_002831.5
• UniProt Accession #: P29350; Q969V8; Q9UK67; A8K306; G3V0F8
• Molecular Weight: 67,561 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 6 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 6
• NCBI Official Symbol: PTPN6
• NCBI Official Synonym Symbols: HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 6; hematopoietic cell phosphatase; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; hematopoietic cell protein-tyrosine phosphatase
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN6
• UniProt Synonym Gene Names: HCP; PTP1C; PTP-1C
• UniProt Entry Name: PTN6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. Ref.20

Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure: Monomer. Interacts with MTUS1

By similarity. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT

By similarity. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.29

Subcellular location: Cytoplasm. Nucleus. Note: In neurons, translocates into the nucleus after treatment with angiotensin II

By similarity. Shuttles between the cytoplasm and nucleus via its association with PDPK1. Ref.26 Ref.29

Tissue specificity: Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Domain: The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation. Ref.31 Ref.33

Post-translational modification: Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation

By similarity. Phosphorylation at Tyr-564 enhances phosphatase activity. Ref.13 Ref.17

Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.Contains 2 SH2 domains.Contains 1 tyrosine-protein phosphatase domain.