Rabbit anti-Human, Mouse PTPN5 Polyclonal Antibody | anti-PTPN5 antibody

PTPN5 (STEP, Tyrosine-protein Phosphatase Non-receptor Type 5, Striatum-enriched Protein-tyrosine Phosphatase, Neural-specific Protein-tyrosine Phosphatase, FLJ14427) (FITC)

Cat. Num. AAA6391410

• Gene Names: PTPN5; STEP; STEP61; PTPSTEP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTPN5; Polyclonal Antibody; PTPN5 (STEP; Tyrosine-protein Phosphatase Non-receptor Type 5; Striatum-enriched Protein-tyrosine Phosphatase; Neural-specific Protein-tyrosine Phosphatase; FLJ14427) (FITC); anti-PTPN5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 565

• Applicable Applications for anti-PTPN5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PTPN5, aa1-565 (NP_008837.1).
• Immunogen Sequence: MNYEGARSERENHAADDSEGGALDMCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQKPPPRGAGSHSLTVRSSLCLFAASQFLLACGVLWFSGYGHIWSQNATNLVSSLLTLLKQLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEPPTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVADFWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTPN5 rabbit polyclonal antibody. Western Blot analysis of PTPN5 expression in mouse lung.)

Western Blot (WB)

(Western Blot analysis of PTPN5 expression in transfected 293T cell line by PTPN5 polyclonal antibody. Lane 1: PTPN5 transfected lysate (63.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PTPN5 antibody

STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537aa predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.

Product Categories/Family for anti-PTPN5 antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 90652859
• NCBI GeneID: 84867
• NCBI Accession #: NP_008837.1
• NCBI GenBank Nucleotide #: NM_006906.1
• UniProt Accession #: P54829
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 5 isoform a
• NCBI Official Synonym Full Names: protein tyrosine phosphatase non-receptor type 5
• NCBI Official Symbol: PTPN5
• NCBI Official Synonym Symbols: STEP; STEP61; PTPSTEP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 5
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 5
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN5
• UniProt Synonym Gene Names: STEP
• UniProt Entry Name: PTN5_HUMAN

Uniprot Description

STEP: a non-receptor protein-tyrosine phosphatase. STEP (striatum enriched phosphatase) Contains a single phosphatase catalytic domain located at the C-terminus. Two alternatively-spliced messages have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: phosphotyrosine binding; protein tyrosine phosphatase activity

Biological Process: protein amino acid dephosphorylation