Rabbit anti-Human PTPN11 Polyclonal Antibody | anti-PTPN11 antibody

PTPN11 (Tyrosine-protein Phosphatase Non-receptor Type 11, Protein-tyrosine Phosphatase 2C, PTP-2C, Protein-tyrosine Phosphatase 1D, PTP-1D, SH-PTP3, SH-PTP2, SHP-2, Shp2, SHPTP2, PTP2C) (MaxLight 550)

Cat. Num. AAA6495781

• Gene Names: PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PTPN11; Polyclonal Antibody; PTPN11 (Tyrosine-protein Phosphatase Non-receptor Type 11; Protein-tyrosine Phosphatase 2C; PTP-2C; Protein-tyrosine Phosphatase 1D; PTP-1D; SH-PTP3; SH-PTP2; SHP-2; Shp2; SHPTP2; PTP2C) (MaxLight 550); anti-PTPN11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPN11.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PTPN11 antibody: FLISA, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to partial human PTPN11.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PTPN11 in 293 cell lysates (2ug/lane) using P9109-29B: Lane 1: nontransfected Lane 2: transiently transfected with the PTPN11 gene.)

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated for 1hr at 37 degree C with MBS629273 (1:25). Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C) as secondary antibody. Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). PTPN11 immunoreactivity is significantly localized to nucleolus and cytoplasm and is weakly localized to the nucleus.)

Related Product Information for anti-PTPN11 antibody

SHP2, also known as PTPN11, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in the gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

Product Categories/Family for anti-PTPN11 antibody

Antibodies; Enzymes; Phosphatase

References

1. Chan, R.J., et al., Blood 105(9):3737-3742 (2005). 2. Sturla, L.M., et al., J. Biol. Chem. 280(15):14597-14604 (2005). 3. Loh, M.L., et al., Leuk. Res. 29(4):459-462 (2005). 4. Wang, Q., et al., J. Biol. Chem. 280(9):8397-8406 (2005). 5. Niihori, T., et al., J. Hum. Genet. 50(4):192-202 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 33356177
• NCBI GeneID: 5781
• NCBI Accession #: NP_002825
• NCBI GenBank Nucleotide #: NM_002834.3
• UniProt Accession #: Q06124; Q96HD7; A8K1D9
• Molecular Weight: 52,828 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: PTPN11
• NCBI Official Synonym Symbols: CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11; PTP-2C; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN11
• UniProt Synonym Gene Names: PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
• UniProt Entry Name: PTN11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; phospholipase binding; SH3/SH2 adaptor activity; peptide hormone receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; phosphoprotein phosphatase activity; D1 dopamine receptor binding; insulin receptor binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; hormone-mediated signaling; activation of MAPK activity; heart development; platelet-derived growth factor receptor signaling pathway; positive regulation of mitotic cell cycle; reproductive process in a multicellular organism; glucose homeostasis; regulation of protein export from nucleus; negative regulation of insulin secretion; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; abortive mitotic cell cycle; inner ear development; negative regulation of cell adhesion mediated by integrin; negative regulation of growth hormone secretion; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of hormone secretion; regulation of multicellular organism growth; negative regulation of cortisol secretion; organ growth; genitalia development; triacylglycerol metabolic process; DNA damage checkpoint; T cell costimulation; insulin receptor signaling pathway; innate immune response; hormone metabolic process; brain development; platelet formation; blood coagulation; leukocyte migration

Disease: Metachondromatosis; Noonan Syndrome 1; Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1