Rabbit PTPN1 Polyclonal Antibody | anti-PTPN1 antibody

PTPN1 Antibody (Center)

Cat. Num. AAA9213782

• Gene Names: PTPN1; PTP1B
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PTPN1; Polyclonal Antibody; PTPN1 Antibody (Center); Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B; PTP1B; anti-PTPN1 antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PTPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human PTPN1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.47 (varies by lot)
• Sequence Positions: 221-249
• Sequence Length: 435

• Applicable Applications for anti-PTPN1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PTPN1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PTPN1 antibody detected the PTPN1 protein (arrow).)

Related Product Information for anti-PTPN1 antibody

The protein encoded by this gene is the founding member of
the protein tyrosine phosphatase (PTP) family, which was isolated
and identified based on its enzymatic activity and amino acid
sequence. PTPs catalyze the hydrolysis of the phosphate monoesters
specifically on tyrosine residues. Members of the PTP family share
a highly conserved catalytic motif, which is essential for the
catalytic activity. PTPs are known to be signaling molecules that
regulate a variety of cellular processes including cell growth,
differentiation, mitotic cycle, and oncogenic transformation. This
PTP has been shown to act as a negative regulator of insulin
signaling by dephosphorylating the phosphotryosine residues of
insulin receptor kinase. This PTP was also reported to
dephosphorylate epidermal growth factor receptor kinase, as well as
JAK2 and TYK2 kinases, which implicated the role of this PTP in
cell growth control, and cell response to interferon stimulation.

Product Categories/Family for anti-PTPN1 antibody

Cardiovascular; Metabolism; Signal transduction

References

Stuible, M., et al. Trends Cell Biol. 20(11):672-679(2010)
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Pradhan, S., et al. J. Biol. Chem. 285(38):29059-29068(2010)
Brasil, A.S., et al. Genet Test Mol Biomarkers 14(3):425-432(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 519666804
• NCBI GeneID: 5770
• NCBI Accession #: NP_001265547.1; NP_002818.1
• NCBI GenBank Nucleotide #: NM_001278618.1
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 49967
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• Protein Family: PTPN13-like protein
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B
• UniProt Entry Name: PTN1_HUMAN

NCBI Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Protein phosphatase, tyrosine (non-receptor); Phosphatase; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding

Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; regulation of signal transduction; negative regulation of insulin receptor signaling pathway; blood coagulation

Disease: Diabetes Mellitus, Noninsulin-dependent