Rabbit PTK6 Polyclonal Antibody | anti-PTK6 antibody

Rabbit Anti PTK6

Cat. Num. AAA225590

• Gene Names: PTK6; BRK
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: Purified IgG was prepared from whole serum by affinity chromatography.
• Synonyms: PTK6; Polyclonal Antibody; Rabbit Anti PTK6; PTK6 antibody; anti-PTK6 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified IgG was prepared from whole serum by affinity chromatography.
• Form/Format: Liquid; Phosphate Buffered Saline
• Sequence Length: 134

• Applicable Applications for anti-PTK6 antibody: Western Blot (WB)
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-451 of human PTK6.
• Preservative Stabilizers: 0.02% Sodium Azide 50% Glycerol
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Western Blot (WB)

(Western blot analysis of various cell and tissue lysates using Rabbit anti PTK6 antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Rabbit anti PTK6 antibody at a 1/100 dilution)

Related Product Information for anti-PTK6 antibody

Rabbit anti PTK6 antibody recognizes protein-tyrosine kinase 6, also known as breast tumor kinase and tyrosine-protein kinase BRK. PTK6 is a nonreceptor tyrosine kinase that is evolutionarily related to the FRK family of kinases and more distantly to the SRC family of tyrosine kinases (Qui and Miller 2002, Brauer and Tyner 2010). It controls various signaling pathways involved in the differentiation and maintenance of normal epithelia (Brauer and Tyner 2010). PTK6 substrates include AKT (Zhang et al. 2005), SLM1 and SLM2 (Haegebarth et al. 2004), STAT3 (Liu et al. 2006), STAT5b (Weaver and Silva 2007). PTK6 is coamplified and coexpressed with ErbB2 in human breast cancers to promote proliferation (Xiang et al. 2008).

NCBI and Uniprot Product Information

• NCBI GI #: 373432692
• NCBI GeneID: 5753
• NCBI Accession #: NP_001243287.1
• NCBI GenBank Nucleotide #: NM_001256358.1
• UniProt Accession #: Q13882
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6
• UniProt Protein Name: Protein-tyrosine kinase 6
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: PTK6
• UniProt Synonym Gene Names: BRK
• UniProt Entry Name: PTK6_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleoplasm; ruffle; extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; protein amino acid autophosphorylation; innate immune response; tyrosine phosphorylation of Stat3 protein; actin cytoskeleton organization and biogenesis; tyrosine phosphorylation of Stat5 protein; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; negative regulation of growth