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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PTGES2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Rabbit PTGES2 Polyclonal Antibody | anti-PTGES2 antibody

PTGES2 Polyclonal Antibody

Gene Names
PTGES2; GBF1; GBF-1; PGES2; C9orf15; mPGES-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
PTGES2; Polyclonal Antibody; PTGES2 Polyclonal Antibody; GBF1; GBF-1; PGES2; C9orf15; mPGES-2; anti-PTGES2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
377
Applicable Applications for anti-PTGES2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PTGES2
Calculated Molecular Weight
42kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PTGES2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PTGES2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using PTGES2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using PTGES2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney cancer using PTGES2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney cancer using PTGES2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using PTGES2 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using PTGES2 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-PTGES2 antibody
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene.
Product Categories/Family for anti-PTGES2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
377
NCBI Official Full Name
Prostaglandin E synthase 2
NCBI Official Synonym Full Names
prostaglandin E synthase 2
NCBI Official Symbol
PTGES2
NCBI Official Synonym Symbols
GBF1; GBF-1; PGES2; C9orf15; mPGES-2
NCBI Protein Information
prostaglandin E synthase 2; mPGE synthase-2; GATE-binding factor 1; prostaglandin-H(2) E-isomerase; microsomal prostaglandin E synthase-2; membrane-associated prostaglandin E synthase 2; membrane-associated prostaglandin E synthase-2; gamma-interferon-activated transcriptional element-binding factor 1
UniProt Protein Name
Prostaglandin E synthase 2
Protein Family
UniProt Gene Name
PTGES2
UniProt Entry Name
PGES2_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

PTGES2: Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. Belongs to the GST superfamily.

Protein type: Isomerase; Membrane protein, integral; Lipid Metabolism - arachidonic acid; EC 5.3.99.3

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: Golgi membrane; mitochondrion; perinuclear region of cytoplasm; integral to membrane; nucleus; cytosol

Molecular Function: glutathione binding; electron carrier activity; DNA binding; lyase activity; protein disulfide oxidoreductase activity; heme binding; prostaglandin-E synthase activity

Biological Process: secretion; cell redox homeostasis; positive regulation of transcription, DNA-dependent; prostaglandin biosynthetic process

Research Articles on PTGES2

Similar Products

Product Notes

The PTGES2 ptges2 (Catalog #AAA9127747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTGES2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTGES2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the PTGES2 ptges2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTGES2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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