Rabbit anti-Mouse PTCHD1 Polyclonal Antibody | anti-PTCHD1 antibody

PTCHD1, ID (PTCHD1, Patched domain-containing protein 1) (AP)

Cat. Num. AAA6338436

• Gene Names: PTCHD1; AUTSX4
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTCHD1; Polyclonal Antibody; ID (PTCHD1; Patched domain-containing protein 1) (AP); Patched domain-containing protein 1; anti-PTCHD1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PTCHD1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTCHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-647 amino acids from the Central region of human PTCHD1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PTCHD1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PTCHD1 antibody detected the PTCHD1 protein (arrow).)

Immunohistochemistry (IHC)

(PTCHD1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTCHD1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-PTCHD1 antibody

Antibodies; Proteins

References

Pinto, D., et al. Nature (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 148271104
• NCBI GeneID: 139411
• NCBI Accession #: NP_775766.2
• NCBI GenBank Nucleotide #: NM_173495.2
• UniProt Accession #: Q96NR3; Q0IJ60; Q6P6B8; B4DQH0
• Molecular Weight: 28,889 Da
• NCBI Official Full Name: patched domain-containing protein 1
• NCBI Official Synonym Full Names: patched domain containing 1
• NCBI Official Symbol: PTCHD1
• NCBI Official Synonym Symbols: AUTSX4
• NCBI Protein Information: patched domain-containing protein 1
• UniProt Protein Name: Patched domain-containing protein 1
• Protein Family: Patched domain-containing protein
• UniProt Gene Name: PTCHD1
• UniProt Entry Name: PTHD1_HUMAN

NCBI Description

This gene encodes a membrane protein with a patched domain. The encoded protein is similar to Drosophila proteins which act as receptors for the morphogen sonic hedgehog. Deletions in this gene, which is located on the X chromosome, are associated with intellectual disability and autism (PMID: 21091464, PMID: 20844286). [provided by RefSeq, Aug 2011]

Uniprot Description

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.4.

Tissue specificity: Widely expressed, including in various regions of the brain with highest expression in the gray and white cerebellum, followed by the cerebellar vermis and the pituitary gland. Ref.4

Sequence similarities: Belongs to the patched family.Contains 1 SSD (sterol-sensing) domain.