Rabbit anti-Human PTBP1 Polyclonal Antibody | anti-PTBP1 antibody

PTBP1, NT (PTBP1, PTB, Polypyrimidine tract-binding protein 1, 57kD RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I) (Biotin)

Cat. Num. AAA6338416

• Gene Names: PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTBP1; Polyclonal Antibody; NT (PTBP1; PTB; Polypyrimidine tract-binding protein 1; 57kD RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I) (Biotin); Heterogeneous nuclear ribonucleoprotein I; anti-PTBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PTBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PTBP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTBP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PTBP1 antibody detected the PTBP1 protein (arrow).)

Related Product Information for anti-PTBP1 antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-PTBP1 antibody

Antibodies; Nuclear Proteins

References

Kanda, T., et al. J. Viral Hepat. 17(9):618-623(2010). Cobbold, L.C., et al. Oncogene 29(19):2884-2891(2010). Verma, B., et al. J. Gen. Virol. 91 (PT 5), 1245-1255 (2010): Maynard, C.M., et al. J. Mol. Biol. 397(1):260-277(2010). Xue, Y., et al. Mol. Cell 36(6):996-1006(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4506243
• NCBI GeneID: 5725
• NCBI Accession #: NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1
• NCBI GenBank Nucleotide #: NM_002819.4
• UniProt Accession #: P26599; Q9BUQ0
• Molecular Weight: 59,633 Da
• NCBI Official Full Name: polypyrimidine tract-binding protein 1 isoform a
• NCBI Official Synonym Full Names: polypyrimidine tract binding protein 1
• NCBI Official Symbol: PTBP1
• NCBI Official Synonym Symbols: PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• NCBI Protein Information: polypyrimidine tract-binding protein 1; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleopro
• UniProt Protein Name: Polypyrimidine tract-binding protein 1
• Protein Family: Polypyrimidine tract-binding protein
• UniProt Gene Name: PTBP1
• UniProt Synonym Gene Names: PTB; PTB; hnRNP I
• UniProt Entry Name: PTBP1_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; membrane; nucleolus

Molecular Function: protein binding; poly-pyrimidine tract binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; negative regulation of muscle cell differentiation; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome