Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using HSD17B1 antibody.)
Rabbit HSD17B1 Polyclonal Antibody | anti-HSD17B1 antibody
HSD17B1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
HSD17B1; Polyclonal Antibody; HSD17B1 Polyclonal Antibody; EDH17B2; EDHB17; HSD17; SDR28C1; anti-HSD17B1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HSD17B1 polyclonal antibody detects endogenous levels of HSD17B1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
328
Applicable Applications for anti-HSD17B1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant protein of human HSD17B1
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using HSD17B1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using HSD17B1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using HSD17B1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using HSD17B1 antibody.)
Related Product Information for anti-HSD17B1 antibody
17b-hydroxysteroid dehydrogenase type 1 (17b-HSD) catalyzes the final step in the formation of estradiol and testosterone from estrone and androstenedione, respectively. Ovarian granulosa cells and breast tissue both express 17b-HSD. Other tissues that express 17b-HSD include testis, placenta, uterus, prostate and adipose tissue. 17b-HSD functions as a homodimer and prefers NADP(H) over NAD(H) for oxidation and reduction. The gene encoding human 17b-HSD maps to chromosome 17q12-q21. The importance of 17b-HSD to estradiol production suggests the specific inhibition of 17b-HSD may aid in breast cancer therapy. Breast cancer patients with an amplification of 17b-HSD expression statistically have a worse outcome than those without. 17b-HSD amplification in tamoxifen-treated patients correlates to decreased breast cancer survival.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35kDa
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 1 isoform 1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 1
Protein Family
UniProt Gene Name
HSD17B1
UniProt Synonym Gene Names
E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD
UniProt Entry Name
DHB1_HUMAN
Uniprot Description
HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q11-q21
Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol
Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity
Biological Process: steroid metabolic process; estrogen metabolic process; steroid biosynthetic process; estrogen biosynthetic process
Similar Products
Product Notes
The HSD17B1 hsd17b1 (Catalog #AAA3010606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the HSD17B1 hsd17b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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