Rabbit anti-Human, Mouse PSTPIP1 Polyclonal Antibody | anti-PSTPIP1 antibody

PSTPIP1 Antibody

Cat. Num. AAA9629663

• Gene Names: PSTPIP1; H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PSTPIP1; Polyclonal Antibody; PSTPIP1 Antibody; CD2 antigen binding protein 1; CD2 binding protein 1; CD2 cytoplasmic tail binding protein; CD2-binding protein 1; CD2BP1; CD2BP1L; CD2BP1S; H PIP; H-PIP; HPIP; PAPAS; PEST phosphatase interacting protein 1; PEST phosphatase-interacting protein 1; PPIP1_HUMAN; Proline serine threonine phosphatase interacting protein 1; Proline-serine-threonine phosphatase-interacting protein 1; PSTPIP; Pstpip1; anti-PSTPIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PSTPIP1 Antibody detects endogenous levels of total PSTPIP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PSTPIP1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The F-BAR domain is important for filament formation. The SH3 domain is not required for filament formation or localization to the uropod.
• Post Translational Modifications: Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV (By similarity).
• Subunit Structure: Homodimer. Homotrimer. Interacts (via coiled-coil domain) with CD2AP, PTPN12 and PTPN18. Interacts (via SH3 domain) with ABL1 and WAS. Interacts (via SH3 and coiled-coil domains) with MEFV (via B-box zinc finger); the interaction allows binding of MEFV to PYCARD and facilitates formation of PYCARD pyroptosomes. Interacts with CD2, DNM2 and FASLG.
• Tissue Specificity: Highly expressed in the peripheral blood leukocytes, granulocytes and monocytes, namely in T-cells and natural killer cells, and in spleen. Weakly expressed in the thymus, small intestine, lung and placenta.
• Function: Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.
• Note: Mainly cytoplasmic in T-cells (PubMed:9857189). Colocalizes in cluster with CD2 near the cell surface membrane in activated T-cells (PubMed:9857189). In monocytes, forms a branched filamentous network in the cytoplasm (PubMed:19584923). In transfected cells, forms relatively straight filaments radiating out from the nucleus (PubMed:19584923). Filament formation requires an intact tubulin cytoskeleton (PubMed:19584923). In migrating neutrophils, colocalizes with PIP5K1C and DNM2 to the trailing edge of the uropod in a actin-dependent manner (PubMed:18480402). Colocalized with PTPN12 in the cytoplasm and the perinuclear region. During interphase, colocalizes with F-actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with CD2AP and WAS in the actin cytoskeleton within the cytoplasm. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PSTPIP1 antibody

Cytoplasm; Cell membrane; Peripheral membrane protein; Cell projection; Uropodium; Cytoskeleton; Perinuclear region; Lamellipodium; Cleavage furrow; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 74739557
• NCBI GeneID: 9051
• UniProt Accession #: O43586; O43585; O95657; B5BU74; B5BUK4
• Molecular Weight: 416
• NCBI Official Full Name: Proline-serine-threonine phosphatase-interacting protein 1
• NCBI Official Synonym Full Names: proline-serine-threonine phosphatase interacting protein 1
• NCBI Official Symbol: PSTPIP1
• NCBI Official Synonym Symbols: H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• NCBI Protein Information: proline-serine-threonine phosphatase-interacting protein 1; CD2-binding protein 1; CD2 antigen-binding protein 1; CD2 cytoplasmic tail-binding protein; PEST phosphatase-interacting protein 1
• UniProt Protein Name: Proline-serine-threonine phosphatase-interacting protein 1
• Protein Family: Proline-serine-threonine phosphatase-interacting protein
• UniProt Gene Name: PSTPIP1
• UniProt Synonym Gene Names: CD2BP1; PEST phosphatase-interacting protein 1
• UniProt Entry Name: PPIP1_HUMAN

NCBI Description

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSTPIP1: an SH3-domain containing protein that may play a role in vesicle formation and transport. Interacts with PTP-PEST to inhibit WASp-driven actin polymerization and synapse formation.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: membrane; lamellipodium; perinuclear region of cytoplasm; contractile ring; stress fiber; uropod; cytosol; cleavage furrow

Molecular Function: protein binding; actin binding; protein phosphatase binding

Biological Process: innate immune response; endocytosis; inflammatory response; signal transduction; cell adhesion

Disease: Pyogenic Sterile Arthritis, Pyoderma Gangrenosum, And Acne