Western Blot (WB)
(Western blot analysis of 293T lysate using PSMG2 antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit PSMG2 Polyclonal Antibody | anti-PSMG2 antibody
PSMG2 Antibody
Gene Names
PSMG2; PAC2; HCCA3; CLAST3; MDS003; HsT1707; TNFSF5IP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PSMG2; Polyclonal Antibody; PSMG2 Antibody; CD40 ligand activated specific transcript 3; CLAST3; HCCA3; Hepatocellular carcinoma susceptibility protein; Hepatocellular carcinoma-susceptibility protein 3; HSPC260; HsT1707; Likely ortholog of mouse CD40 ligand activated specific transcript 3; MDS003; PAC-2; PAC2; proteasome (prosome; macropain) assembly chaperone 2; Proteasome assembly chaperone 2; psmG2; PSMG2_HUMAN; TNFSF5IP1; Tumor necrosis factor superfamily member 5-induced protein 1; anti-PSMG2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PSMG2 antibody detects endogenous levels of total PSMG2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
264
Applicable Applications for anti-PSMG2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human PSMG2
Subcellular Location
Nucleus.
Tissue Specificity
Widely expressed with highest levels in lung, brain and colon. Moderately expressed in muscle, stomach, spleen and heart. Weakly expressed in small intestine, pancreas and liver. Highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (88%), Sheep (88%), Rabbit (86%), Dog (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of 293T lysate using PSMG2 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of 293T lysate using PSMG2 antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9606780 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606780 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PSMG2 antibody
Description: PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Function: Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Subunit Structure: Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits.
Post-translational Modifications: Degraded by the proteasome upon completion of 20S proteasome maturation.
Similarity: Belongs to the PSMG2 family.
Function: Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Subunit Structure: Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits.
Post-translational Modifications: Degraded by the proteasome upon completion of 20S proteasome maturation.
Similarity: Belongs to the PSMG2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 29 kDa
Predicted: 30 kDa
Predicted: 30 kDa
NCBI Official Full Name
proteasome assembly chaperone 2 isoform 1
NCBI Official Synonym Full Names
proteasome assembly chaperone 2
NCBI Official Symbol
PSMG2
NCBI Official Synonym Symbols
PAC2; HCCA3; CLAST3; MDS003; HsT1707; TNFSF5IP1
NCBI Protein Information
proteasome assembly chaperone 2
UniProt Protein Name
Proteasome assembly chaperone 2
Protein Family
UniProt Gene Name
PSMG2
UniProt Synonym Gene Names
PAC-2
Uniprot Description
Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Research Articles on PSMG2
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Product Notes
The PSMG2 psmg2 (Catalog #AAA9606780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMG2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSMG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PSMG2 psmg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMG2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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