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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Hela and HEPG2 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 35 seconds)

Rabbit anti-Human PSMF1 Polyclonal Antibody | anti-PSMF1 antibody

PSMF1 Antibody

Gene Names
PSMF1; PI31
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
PSMF1; Polyclonal Antibody; PSMF1 Antibody; PI31; anti-PSMF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total PSMF1 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.1 mg/ml (varies by lot)
Sequence Length
271
Applicable Applications for anti-PSMF1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunogen
Full length fusion protein of human PSMF1
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Hela and HEPG2 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 35 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Hela and HEPG2 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 35 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/30,)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/30,)
Related Product Information for anti-PSMF1 antibody
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.
Product Categories/Family for anti-PSMF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,817 Da
NCBI Official Full Name
proteasome inhibitor PI31 subunit
NCBI Official Synonym Full Names
proteasome inhibitor subunit 1
NCBI Official Symbol
PSMF1
NCBI Official Synonym Symbols
PI31
NCBI Protein Information
proteasome inhibitor PI31 subunit
UniProt Protein Name
Proteasome inhibitor PI31 subunit
Protein Family
UniProt Gene Name
PSMF1
UniProt Synonym Gene Names
hPI31
UniProt Entry Name
PSMF1_HUMAN

Uniprot Description

PSMF1: Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. Belongs to the proteasome inhibitor PI31 family.

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cytosol; endoplasmic reticulum; membrane; nucleoplasm; perinuclear region of cytoplasm

Molecular Function: protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway

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Product Notes

The PSMF1 psmf1 (Catalog #AAA9416361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the PSMF1 psmf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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