Rabbit anti-Human PSMD2 Polyclonal Antibody | anti-PSMD2 antibody

PSMD2 Antibody

Cat. Num. AAA7001747

• Gene Names: PSMD2; S2; P97; RPN1; TRAP2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%,Protein G purified
• Synonyms: PSMD2; Polyclonal Antibody; PSMD2 Antibody; 26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; Protein 55.11; Tumor necrosis factor type 1 receptor-associated protein 2; TRAP2; anti-PSMD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 778

• Applicable Applications for anti-PSMD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant human 26S proteasome non-ATPase regulatory subunit 2 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68352
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,U251 whole cell lysateAll lanes: PSMD2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 101,83,86 kDaObserved band size: 100,40 kDa)

Immunohistochemistry (IHC)

(IHC image of MBS7001747 diluted at 1:1000 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7001747 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PSMD2 antibody

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 523425734
• NCBI GeneID: 5708
• NCBI Accession #: NP_001265637.1
• NCBI GenBank Nucleotide #: NM_001278708.1
• UniProt Accession #: Q13200; Q12932; Q15321; Q53XQ4; Q96I12; B4DX07; B4DXY1; E7EW34; E9PCS3
• Molecular Weight: 85,606 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 2 isoform 2
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 2
• NCBI Official Symbol: PSMD2
• NCBI Official Synonym Symbols: S2; P97; RPN1; TRAP2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 2
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 2
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD2
• UniProt Synonym Gene Names: TRAP2
• UniProt Entry Name: PSMD2_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

PSMD2: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S2 family.

Protein type: Cell cycle regulation; Proteasome complex

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: cytosol; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle

Molecular Function: endopeptidase activity; enzyme regulator activity; protein binding

Biological Process: activation of MAPKK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptosis; axon guidance; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; gene expression; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; mitotic cell cycle; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; nerve growth factor receptor signaling pathway; polyamine metabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; programmed cell death; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; Ras protein signal transduction; regulation of amino acid metabolic process; regulation of apoptosis; regulation of mRNA stability; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction