Proteinase K-lyophilization Protein

Proteinase K-lyophilization

• Purity: >=95% by SDS-PAGE
• Synonyms: Proteinase K-lyophilization; Proteinase K; Endopeptidase K; Tritirachium alkaline proteinase; Proteinase K-lyophilization protein

• Host: Yeast
• Purity/Purification: >=95% by SDS-PAGE
• Form/Format: Lyophilized powder

• Source: Tritirachium album Limber
• Activity: >=30U/mg. One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH7.5 at 37 degree C.
• EC Number: 3.4.21.14
• Preparation and Storage: The shelf life of the lyophilized form is 24 months at -20 degree C/-80 degree C, 18 months at 4 degree C, and 12 months at room temperature.; Enzyme activity maintains above 95% when it store at 4 degree C for 1 year

Application Data

(Detect RNase residue by agarose gel electrophores)

Application Data

(Detect DNase residue by agarose gel electrophores)

Application Data

(Detect Nucleic acid residue by agarose gel electrophores)

Application Data

(The proteinase K was stored at 4 degree C for one year. Enzymetic activities were asaayed every month during one year.As shown in Fig.3, it indicates that proteinase K is stable and enzyme activity is maintained more than 95%.)

Application Data

(According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating.)

Activity

(Activity measurements were compared to from three other vendors. Proteinase K from us is better than other vendors.)

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.The purity and the molecular weight of our proteinase K were over 95% and 28.9 kDa by SDS-PAGE.)

Related Product Information for Proteinase K-lyophilization protein

Proteinase K is a serine protease that is used to digest proteins and remove contamination from nucleic acid preparations. In molecular biology research, adding Proteinase K to nucleic acid preparations inactivates nucleases that could degrade DNA or RNA during isolation and purification applications.