Rabbit anti-Human, Mouse SMC1A Polyclonal Antibody | anti-SMC1A antibody

SMC1A Rabbit Polyclonal

Cat. Num. AAA7604249

• Gene Names: SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: SMC1A; Polyclonal Antibody; SMC1A Rabbit Polyclonal; anti-SMC1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1211

• Applicable Applications for anti-SMC1A antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Structural maintenance of chromosomes 1A
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gliomas tissue slide using MBS7604249 (SMC1A antibody) at dilution of 1:200)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using MBS7604249 (SMC1A antibody) at dilution of 1:50)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7604249 (SMC1A antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 527317371
• NCBI GeneID: 8243
• NCBI Accession #: NP_001268392.1
• NCBI GenBank Nucleotide #: NM_001281463.1
• Molecular Weight: 143,233 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 1A isoform 2
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 1A
• NCBI Official Symbol: SMC1A
• NCBI Official Synonym Symbols: SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• NCBI Protein Information: structural maintenance of chromosomes protein 1A
• UniProt Protein Name: Structural maintenance of chromosomes protein 1A
• Protein Family: Structural maintenance of chromosomes protein
• UniProt Gene Name: SMC1A
• UniProt Synonym Gene Names: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
• UniProt Entry Name: SMC1A_HUMAN

NCBI Description

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

Protein type: DNA replication; RNA splicing

Chromosomal Location of Human Ortholog: Xp11.22-p11.21

Cellular Component: chromosome; chromosome, pericentric region; cohesin core heterodimer; condensed nuclear chromosome; cytoplasm; cytosol; kinetochore; nucleoplasm; nucleus

Molecular Function: ATP binding; chromatin binding; protein binding; protein heterodimerization activity

Biological Process: cell division; DNA damage response, signal transduction; DNA repair; meiosis; mitotic cell cycle checkpoint; mitotic sister chromatid cohesion; mitotic sister chromatid segregation; mitotic spindle organization and biogenesis; negative regulation of DNA endoreduplication; protein sumoylation; response to radiation; sister chromatid cohesion; stem cell maintenance

Disease: Cornelia De Lange Syndrome 2