Rabbit PSMD10 Polyclonal Antibody | anti-PSMD10 antibody

PSMD10 (Proteasome 26S Subunit, Non ATPase 10, p28, p28(GANK), dJ889N15.2) (HRP)

Cat. Num. AAA6433683

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PSMD10; Polyclonal Antibody; PSMD10 (Proteasome 26S Subunit; Non ATPase 10; p28; p28(GANK); dJ889N15.2) (HRP); anti-PSMD10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMD10. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMD10 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMD10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of SW620 cell lines, using PSMD10 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using PSMD10 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-PSMD10 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 20532206
• NCBI GeneID: 5716
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 226
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10; gankyrin; ankyrin repeat protein; 26S proteasome regulatory subunit p28; hepatocellular carcinoma-associated protein p28-II
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10
• UniProt Entry Name: PSD10_HUMAN

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

PSMD10: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: proteasome complex; nucleoplasm; intermediate filament cytoskeleton; proteasome regulatory particle; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; protein polyubiquitination; viral reproduction; apoptosis; negative regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; antigen processing and presentation of peptide antigen via MHC class I; cytoplasmic sequestering of NF-kappaB; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis