Rabbit PSMC6 Polyclonal Antibody | anti-PSMC6 antibody

PSMC6 Polyclonal Antibody

Cat. Num. AAA3006112

• Gene Names: PSMC6; p42; SUG2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PSMC6; Polyclonal Antibody; PSMC6 Polyclonal Antibody; 26S protease regulatory subunit 10B; 26S proteasome AAA-ATPase subunit RPT4; Proteasome 26S subunit ATPase 6; Proteasome subunit p42; SUG2; anti-PSMC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PSMC6 polyclonal antibody detects endogenous levels of PSMC6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 403

• Applicable Applications for anti-PSMC6 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 107-152 of Human PSMC6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PSMC6 pAb at 1:1000 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:Hela whole cell lysate (40ug))

Related Product Information for anti-PSMC6 antibody

In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. PSMC6 (proteasome (prosome, macropain) 26S subunit, ATPase 6), also known as P44, p42, SUG2, S10B or CADP44, is a regulatory component of the 26S proteasome. More specifically, PSMC6 is one of the six ATPase subunits of the 19S regulator base. It contains a leucine zipper motif and an AAA (ATPase associated with diverse cellular activities) domain and belongs to the AAA ATPase family of chaperone-like ATPases.

NCBI and Uniprot Product Information

• NCBI GI #: 195539395
• NCBI GeneID: 5706
• NCBI Accession #: NP_002797.3
• NCBI GenBank Nucleotide #: NP_002797.3
• UniProt Accession #: P62333
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: 26S proteasome regulatory subunit 10B
• NCBI Official Synonym Full Names: proteasome 26S subunit, ATPase 6
• NCBI Official Symbol: PSMC6
• NCBI Official Synonym Symbols: p42; SUG2
• NCBI Protein Information: 26S proteasome regulatory subunit 10B
• UniProt Protein Name: 26S protease regulatory subunit 10B
• Protein Family: 26S protease regulatory
• UniProt Gene Name: PSMC6
• UniProt Synonym Gene Names: SUG2
• UniProt Entry Name: PRS10_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT4: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 14q22.1

Cellular Component: nucleoplasm; proteasome complex; membrane; nucleus; cytosol

Molecular Function: protein binding, bridging; protein binding; ATPase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis