Rabbit anti-Mouse Apoptosis Signal Regulating Kinase 1 (ASK1) Polyclonal Antibody | anti-ASK1 antibody

Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1)

Cat. Num. AAA2016169

• Gene Names: Map3k5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Apoptosis Signal Regulating Kinase 1 (ASK1); Polyclonal Antibody; Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1); anti-ASK1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ASK1. It has been selected for its ability to recognize ASK1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE
• Sequence Length: 1374

• Applicable Applications for anti-ASK1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant ASK1 (Leu693~Arg933) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2054288
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ASK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Heart Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 171846249
• NCBI GeneID: 26408
• NCBI Accession #: NP_032606.4
• NCBI GenBank Nucleotide #: NM_008580.4
• UniProt Accession #: O35099; Q14AY5
• Molecular Weight: 154,512 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: Map3k5
• NCBI Official Synonym Symbols: ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: DASH complex
• UniProt Gene Name: Map3k5
• UniProt Synonym Gene Names: Ask1; Mekk5; ASK-1; MEK kinase 5; MEKK 5

Uniprot Description

ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 10|10 A3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; external side of plasma membrane; IRE1-TRAF2-ASK1 complex; protein complex

Molecular Function: ATP binding; identical protein binding; kinase activity; magnesium ion binding; MAP kinase kinase kinase activity; metal ion binding; nucleotide binding; protein binding; protein domain specific binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; apoptosis; cellular response to amino acid starvation; cellular response to hydrogen peroxide; cellular response to reactive nitrogen species; immune system process; induction of apoptosis by oxidative stress; innate immune response; JNK cascade; MAPK cascade; p38MAPK cascade; phosphorylation; positive regulation of apoptosis; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of myoblast differentiation; positive regulation of neuron death; positive regulation of p38MAPK cascade; positive regulation of protein phosphorylation; positive regulation of transcription, DNA-templated; positive regulation of vascular smooth muscle cell proliferation; programmed necrotic cell death; protein amino acid phosphorylation; regulation of cell death; stress-activated MAPK cascade