Rabbit PSMB5 Polyclonal Antibody | anti-PSMB5 antibody

PSMB5 (Proteasome Subunit beta Type-5, X, MB1, LMPX) (PE)

Cat. Num. AAA6433601

• Gene Names: PSMB5; X; MB1; LMPX
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PSMB5; Polyclonal Antibody; PSMB5 (Proteasome Subunit beta Type-5; X; MB1; LMPX) (PE); anti-PSMB5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMB5. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMB5 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMB5.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMB5 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using PSMB5 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-PSMB5 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 187608890
• NCBI GeneID: 5693
• UniProt Accession #: P28074; Q16242; Q6PEW2; Q7Z3B5; Q86T01; Q9TNN9; B2R4N9; B4DUM9; D3DS43; E9PAV2
• Molecular Weight: Calculated MW: 17kDa, 21kDa, 28kDa; Observed MW: 21kDa
• NCBI Official Full Name: Proteasome subunit beta type-5
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 5
• NCBI Official Symbol: PSMB5
• NCBI Official Synonym Symbols: X; MB1; LMPX
• NCBI Protein Information: proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large mul
• UniProt Protein Name: Proteasome subunit beta type-5
• Protein Family: Proteasome
• UniProt Gene Name: PSMB5
• UniProt Synonym Gene Names: LMPX; MB1; X
• UniProt Entry Name: PSB5_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle