Rabbit anti-Human PSMA5 Polyclonal Antibody | anti-PSMA5 antibody

PSMA5, ID (Proteasome Subunit alpha Type-5, Proteasome Zeta Chain, Macropain Zeta Chain, Multicatalytic Endopeptidase Complex Zeta Chain) (PE)

Cat. Num. AAA6494909

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMA5; Polyclonal Antibody; ID (Proteasome Subunit alpha Type-5; Proteasome Zeta Chain; Macropain Zeta Chain; Multicatalytic Endopeptidase Complex Zeta Chain) (PE); anti-PSMA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMA5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PSMA5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human PSMA5 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of PSMA5 Antibody (Center) in HL60 cell line lysates (35ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PSMA5 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 23110942
• NCBI GeneID: 5686
• NCBI Accession #: NP_002781
• NCBI GenBank Nucleotide #: NM_002790.3
• UniProt Accession #: P28066; Q3T1C1; Q6IBF7; B2R8F6; B4E2V4
• Molecular Weight: 30.5kDa (277aa), confirmed by MALDI-TOF
• NCBI Official Full Name: proteasome subunit alpha type-5 isoform 1
• UniProt Protein Name: Proteasome subunit alpha type-5
• UniProt Gene Name: PSMA5
• UniProt Entry Name: PSA5_HUMAN

Uniprot Description

PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle