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Western Blot (WB) (Western blot analysis of PSA antibody (C-term) in MCF-7 cell line lysates (35ug/lane). PSA (arrow) was detected using the purified Pab.)

Rabbit anti-Human PSA Polyclonal Antibody | anti-NPEPPS antibody

PSA Antibody (C-term)

Gene Names
NPEPPS; PSA; AAP-S; MP100
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PSA; Polyclonal Antibody; PSA Antibody (C-term); Puromycin-sensitive aminopeptidase; Cytosol alanyl aminopeptidase; AAP-S; NPEPPS; anti-NPEPPS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 709-737 amino acids from the C-terminal region of human PSA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
709-737
Sequence Length
839
Applicable Applications for anti-NPEPPS antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of PSA antibody (C-term) in MCF-7 cell line lysates (35ug/lane). PSA (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PSA antibody (C-term) in MCF-7 cell line lysates (35ug/lane). PSA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PSA Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PSA Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-NPEPPS antibody
PSA is the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
Product Categories/Family for anti-NPEPPS antibody
References
Thompson,M.W., Peptides 24 (9), 1359-1365 (2003)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
103276
NCBI Official Full Name
puromycin-sensitive aminopeptidase
NCBI Official Synonym Full Names
aminopeptidase puromycin sensitive
NCBI Official Symbol
NPEPPS
NCBI Official Synonym Symbols
PSA; AAP-S; MP100
NCBI Protein Information
puromycin-sensitive aminopeptidase
UniProt Protein Name
Puromycin-sensitive aminopeptidase
Protein Family
UniProt Gene Name
NPEPPS
UniProt Synonym Gene Names
PSA; PSA; AAP-S
UniProt Entry Name
PSA_HUMAN

NCBI Description

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

NPEPPS: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. Belongs to the peptidase M1 family.

Protein type: Protease; EC 3.4.11.14

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytosol; nucleus

Molecular Function: zinc ion binding; metallopeptidase activity; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; proteolysis

Research Articles on NPEPPS

Similar Products

Product Notes

The NPEPPS npepps (Catalog #AAA9202448) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 709-737. The PSA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the NPEPPS npepps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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