Puromycin Sensitive Aminopeptidase (PSA) Recombinant Protein | PSA recombinant protein

Recombinant Puromycin Sensitive Aminopeptidase (PSA)

Cat. Num. AAA2010765

• Gene Names: NPEPPS; PSA; AAP-S; MP100
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Puromycin Sensitive Aminopeptidase (PSA); Recombinant Puromycin Sensitive Aminopeptidase (PSA); PSA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-GTVGFYR TQYSSAMLES LLPGIRDLSL PPVDRLGLQN DLFSLARAGI ISTVEVLKVM EAFVNEPNYT VWSDLSCNLG ILSTLLSHTD FYEEIQEFVK DVFSPIGERL GWDPKPGEGH LDALLRGLVL GKLGKAGHKA TLEEARRRFK DHVEGKQILS ADLRSPVYLT VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE RVLGATLLPD LIQ
• Sequence Length: 919

• Applicable Applications for PSA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gly584~Gln793 (Accession # P55786) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 158937236
• NCBI GeneID: 9520
• NCBI Accession #: NP_006301.3
• NCBI GenBank Nucleotide #: NM_006310.3
• UniProt Accession #: P55786; Q6P145; Q9NP16; Q9UEM2
• Molecular Weight: 24.9kDa
• NCBI Official Full Name: puromycin-sensitive aminopeptidase
• NCBI Official Synonym Full Names: aminopeptidase puromycin sensitive
• NCBI Official Symbol: NPEPPS
• NCBI Official Synonym Symbols: PSA; AAP-S; MP100
• NCBI Protein Information: puromycin-sensitive aminopeptidase; metalloproteinase MP100; cytosol alanyl aminopeptidase
• UniProt Protein Name: Puromycin-sensitive aminopeptidase
• UniProt Gene Name: NPEPPS
• UniProt Synonym Gene Names: PSA; PSA; AAP-S
• UniProt Entry Name: PSA_HUMAN

NCBI Description

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

NPEPPS: Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. Belongs to the peptidase M1 family.

Protein type: EC 3.4.11.14; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleus; cytosol

Molecular Function: metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; proteolysis