Western Blot (WB)
(Western Blot: The whole cell lysates derived from LNCap (lane 1) and HEK293 (lane 2) were immunoblotted by Rabbit anti-PSA (MBS462078) at 1:1000.)
Rabbit anti-Human PSA Polyclonal Antibody | anti-PSA antibody
Rabbit anti PSA Polyclonal antibody
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope-Affinity Purification.
Synonyms
PSA; Polyclonal Antibody; Rabbit anti PSA Polyclonal antibody; Rabbit anti-PSA; Prostate Specific Antigen (PSA); Kallikrein 3 (KLK3) APS; hK3; KLK2A1;; anti-PSA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This antibody recognizes ~27 kDa of human PSA protein. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope-Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-PSA antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to internal sequence of human PSA protein.
Positive Control
LNCaP cell lysate
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot (WB)
(Western Blot: The whole cell lysates derived from LNCap (lane 1) and HEK293 (lane 2) were immunoblotted by Rabbit anti-PSA (MBS462078) at 1:1000.)
Western Blot (WB)
(Western Blot: The whole cell lysates derived from LNCap (lane 1) and HEK293 (lane 2) were immunoblotted by Rabbit anti-PSA (MBS462078) at 1:1000.)
Immunohistochemistry (IHC)
(IHC: Human prostate Tissue is stained by rabbit anti PSA MBS462078 at 1:200 (RT, 10min.) Staining of formalin-fixed tissue requires boiling tissue section in 10mM Citrate Buffer, pH 6.0 for 10min followed by cooling at RT for 20mins.)
Immunohistochemistry (IHC)
(IHC: Human prostate Tissue is stained by rabbit anti PSA MBS462078 at 1:200 (RT, 10min.) Staining of formalin-fixed tissue requires boiling tissue section in 10mM Citrate Buffer, pH 6.0 for 10min followed by cooling at RT for 20mins.)
Related Product Information for anti-PSA antibody
Prostate specific antiben (PSA), also called kallikrein-3, is a chymotrypsin-like serine protease exclusively produced by the prostate epithelium, and abundant in seminal fluid. The PSA is an established tumor marker that aids in the identification, staging and follow up of prostate cancer. PSA can be detected in the sera of patients with prostatic carcinoma.
References
Weiner GJ, et al. Prostate cell surface antigen-specific antibodies. US Patent 6,207,805 dated Mar 27 2001 Rokhlin OW, et al. a prostate-specific surface-reactive monoclonal antibody. Cancer Lett. 131: 129-136, 1998.
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Product Notes
The PSA (Catalog #AAA462078) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti PSA Polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the PSA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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