Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated ? at 1:400 overnight at 4°C)
Rabbit Prune homolog 2/C9orf65 Polyclonal Antibody | anti-C9orf65 antibody
Prune homolog 2/C9orf65 Polyclonal Antibody
Gene Names
PRUNE2; BMCC1; BNIPXL; C9orf65; KIAA0367; A214N16.3; bA214N16.3; RP11-58J3.2
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
Prune homolog 2/C9orf65; Polyclonal Antibody; Prune homolog 2/C9orf65 Polyclonal Antibody; A214N16 3; BCH motif containing molecule at the carboxyl terminal region 1; BMCC1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIPXL; C9orf65; chromosome 9 open reading frame 65; prune homolog 2 (Drosophila); Prune2.; anti-C9orf65 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
Clonality
Polyclonal
Specificity
Prune homolog 2/C9orf65 Polyclonal Antibody detects endogenous levels of Prune homolog 2/C9orf65 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 95% Glycerol.
Applicable Applications for anti-C9orf65 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human Prune homolog 2/C9orf56:2801-2900/3088
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -14 degree C long term. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated ? at 1:400 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated ? at 1:400 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)
Related Product Information for anti-C9orf65 antibody
The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
340,635 Da
NCBI Official Full Name
protein prune homolog 2
NCBI Official Synonym Full Names
prune homolog 2 (Drosophila)
NCBI Official Symbol
PRUNE2
NCBI Official Synonym Symbols
BMCC1; BNIPXL; C9orf65; KIAA0367; A214N16.3; bA214N16.3; RP11-58J3.2
NCBI Protein Information
protein prune homolog 2; olfaxin; truncated PRUNE2; BNIP2 motif-containing molecule at the C-terminal region 1; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2 motif containing molecule at the carboxyl terminal region 1
UniProt Protein Name
Protein prune homolog 2
UniProt Gene Name
PRUNE2
UniProt Synonym Gene Names
BMCC1; BNIPXL; C9orf65; KIAA0367
UniProt Entry Name
PRUN2_HUMAN
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Product Notes
The C9orf65 prune2 (Catalog #AAA3015376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prune homolog 2/C9orf65 Polyclonal Antibody reacts with Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Prune homolog 2/C9orf65 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the C9orf65 prune2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prune homolog 2/C9orf65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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