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Western Blot (WB) (Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.)

Rabbit anti-Human, Mouse PRTN3 Polyclonal Antibody | anti-PRTN3 antibody

PRTN3 Antibody

Gene Names
PRTN3; MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
PRTN3; Polyclonal Antibody; PRTN3 Antibody; ACPA antibody; AGP 7 antibody; AGP7 antibody; AGP7 serine proteinase antibody; Azurophil Granule Protein 7 antibody; C ANCA antibody; C ANCA antigen antibody; C-ANCA antigen antibody; CANCA antibody; EC 3.4.21.76 antibody; Leukocyte proteinase 3 antibody; MBN antibody; MBT antibody; MBT WEGENER AUTOANTIGEN antibody; Myeloblastin antibody; Neutrophil proteinase 4 antibody; NP 4 antibody; NP-4 antibody; NP4 antibody; P29 antibody; PR 3 antibody; PR-3 antibody; PR3 antibody; Proteinase 3 antibody; Proteinase3 antibody; PRTN 3 antibody; Prtn3 antibody; PRTN3_HUMAN antibody; Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody; Serine proteinase; neutrophil antibody; Wegener autoantigen antibody; Wegener granulomatosis autoantigen antibody; anti-PRTN3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PRTN3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
WB: 1:500
IHC: 1:100-1:500
ICC: 1:50-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.)

Western Blot (WB)

(Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution.)

Western Blot (WB) (Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining PRTN3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PRTN3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining PRTN3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PRTN3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining PRTN3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining PRTN3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-PRTN3 antibody
The major features of Wegener granulomatosis are necrotizing granulomatous lesions, which most often affect the upper and lower airways and are associated with vasculitis, necrotizing glomerulonephritis and pulmonary capillaritis. The antigen responsible for this disease is Proteinase 3 (PR3, P29 or myeloblastin), which is one of the antibiotic proteins of neutrophilic granules belonging to the serine protease family. It is closely related to two others: Neutrophil Elastase and azurocidin. All three genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. PR3 is a neutrophil protein which is able to cleave elastin and is involved in proliferation of human leukemia cells. PR3 is expressed specifically in immature myeloid cells and is a G-CSF-responsive protein critical to factor-independent growth. The genes for all three of the related serine protease family members are located in a cluster on the tip of the short arm of human chromosome 19.
Product Categories/Family for anti-PRTN3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,807 Da
NCBI Official Full Name
myeloblastin
NCBI Official Synonym Full Names
proteinase 3
NCBI Official Symbol
PRTN3
NCBI Official Synonym Symbols
MBN; MBT; NP4; P29; PR3; ACPA; AGP7; NP-4; PR-3; CANCA; C-ANCA
NCBI Protein Information
myeloblastin; C-ANCA antigen; wegener autoantigen; leukocyte proteinase 3; neutrophil proteinase 4; azurophil granule protein 7; serine proteinase, neutrophil; Wegener granulomatosis autoantigen
UniProt Protein Name
Myeloblastin
Protein Family
UniProt Gene Name
PRTN3
UniProt Synonym Gene Names
MBN; PR-3; PR3; NP-4
UniProt Entry Name
PRTN3_HUMAN

Uniprot Description

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; Cell cycle regulation; EC 3.4.21.76

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol

Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of phagocytosis; collagen catabolic process; positive regulation of cell proliferation; blood coagulation; proteolysis

Research Articles on PRTN3

Similar Products

Product Notes

The PRTN3 prtn3 (Catalog #AAA9435362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRTN3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRTN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:500 IHC: 1:100-1:500 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the PRTN3 prtn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRTN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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