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Immunohistochemistry (IHC) (Anti-APOM / Apolipoprotein M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Goat APOM / Apolipoprotein M Polyclonal Antibody | anti-APOM antibody

Goat Polyclonal to Mouse APOM / Apolipoprotein M

Gene Names
APOM; G3a; NG20; apo-M; HSPC336
Reactivity
Human, Mouse
Predicted Reactivity: Rat (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
APOM / Apolipoprotein M; Polyclonal Antibody; Goat Polyclonal to Mouse APOM / Apolipoprotein M; Anti-APOM / Apolipoprotein M Antibody (Internal) IHC-plus; APOM; Alternative name: G3a; NG20; Apo-M; Apolipoprotein M; HSPC336; G3a; NG20-like protein; Protein G3a; Mouse APOM; anti-APOM antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human, Mouse
Predicted Reactivity: Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Mouse APOM / Apolipoprotein M.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
188
Applicable Applications for anti-APOM antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in litera...
Target Species
Mouse
Immunogen Description
Synthetic peptide C-KGNMELRTEGRPD from an internal region of mouse APOM / Apolipoprotein M (NP_061286.1). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (92%).
Immunogen Type
Synthetic peptide
Immunogen
APOM / Apolipoprotein M antibody was raised against synthetic peptide C-KGNMELRTEGRPD from an internal region of mouse APOM / Apolipoprotein M (NP_061286.1). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (92%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-APOM / Apolipoprotein M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC) (Anti-APOM / Apolipoprotein M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(APOM antibody (0.5 ug/ml) staining of human placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) (APOM antibody (0.5 ug/ml) staining of human placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,051 Da
NCBI Official Full Name
apolipoprotein M isoform 1
NCBI Official Synonym Full Names
apolipoprotein M
NCBI Official Symbol
APOM
NCBI Official Synonym Symbols
G3a; NG20; apo-M; HSPC336
NCBI Protein Information
apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20
UniProt Protein Name
Apolipoprotein M
Protein Family
UniProt Gene Name
APOM
UniProt Synonym Gene Names
G3A; NG20; Apo-M; ApoM
UniProt Entry Name
APOM_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: integral to plasma membrane

Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process

Research Articles on APOM

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Product Notes

The APOM apom (Catalog #AAA244104) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Mouse APOM / Apolipoprotein M reacts with Human, Mouse Predicted Reactivity: Rat (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's APOM / Apolipoprotein M can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.3 - 1 ug/ml) Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in litera.. Researchers should empirically determine the suitability of the APOM apom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOM / Apolipoprotein M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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