Rabbit anti-Human PRSS2 Polyclonal Antibody | anti-PRSS2 antibody

PRSS2, ID (PRSS2, TRY2, TRYP2, Trypsin-2, Anionic trypsinogen, Serine protease 2, Trypsin II) (Azide free) (HRP)

Cat. Num. AAA6337912

• Gene Names: PRSS2; TRY2; TRY8; TRYP2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PRSS2; Polyclonal Antibody; ID (PRSS2; TRY2; TRYP2; Trypsin-2; Anionic trypsinogen; Serine protease 2; Trypsin II) (Azide free) (HRP); Trypsin II; anti-PRSS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRSS2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRSS2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 80-108 in the central region of human PRSS2, Swiss/UniProt Accession: P07478.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6004781. This demonstrates that MBS6004781 detected the PRSS2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6004781 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004781 for immunohistochemistry.)

Product Categories/Family for anti-PRSS2 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Felderbauer, P., et al. Exp. Clin. Endocrinol. Diabetes (2010). In press 2. Gao, J., et al. Oncol. Rep. 23(6):1683-1692(2010). 3. Cevik, Y., et al. Ulus Travma Acil Cerrahi Derg 16(2):125-129(2010). 4. Andersen, A.M., et al. Pancreas 39(1):26-30(2010). 5. Johansen, D., et al. Pancreatology 10 (2-3), 229-237 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4506147
• NCBI GeneID: 5645
• NCBI Accession #: NP_002761.1
• NCBI GenBank Nucleotide #: NM_002770.3
• UniProt Accession #: P07478
• Molecular Weight: 26,488 Da
• NCBI Official Full Name: trypsin-2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: serine protease 2
• NCBI Official Symbol: PRSS2
• NCBI Official Synonym Symbols: TRY2; TRY8; TRYP2
• NCBI Protein Information: trypsin-2
• UniProt Protein Name: Trypsin-2
• Protein Family: Trypsin
• UniProt Gene Name: PRSS2
• UniProt Synonym Gene Names: TRY2; TRYP2
• UniProt Entry Name: TRY2_HUMAN

NCBI Description

This gene belongs to the trypsin family of serine proteases and encodes anionic trypsinogen. It is part of a cluster of trypsinogen genes that are located within the T cell receptor beta locus. Enzymes of this family cleave peptide bonds that follow lysine or arginine residues. This protein is found at high levels in pancreatic juice and its upregulation is a characteristic feature of pancreatitis. This protein has also been found to activate pro-urokinase in ovarian tumors, suggesting a function in tumor invasion. In addition, this enzyme is able to cleave across the type II collagen triple helix in rheumatoid arthritis synovitis tissue, potentially participating in the degradation of type II collagen-rich cartilage matrix. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2015]

Uniprot Description

trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide; Calcium-binding; EC 3.4.21.4; Protease; Cell adhesion

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; digestion; innate immune response; proteolysis; positive regulation of cell growth

Disease: Pancreatitis, Hereditary