Western Blot (WB)
(Mouse Prr5 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Prr5 antibody detected the Prr5 protein (arrow).)
Rabbit anti-Mouse Prr5 Polyclonal Antibody | anti-Prr5 antibody
Prr5, CT (Prr5, Protor1, Proline-rich protein 5, Protein observed with Rictor-1) (Azide free) (HRP)
Gene Names
Prr5; C78947; Arhgap8; Protor1; AU043908; Protor-1; C030017C09Rik
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Prr5; Polyclonal Antibody; CT (Prr5; Protor1; Proline-rich protein 5; Protein observed with Rictor-1) (Azide free) (HRP); Protein observed with Rictor-1; anti-Prr5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-Prr5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Prr5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of mouse Prr5.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Mouse Prr5 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Prr5 antibody detected the Prr5 protein (arrow).)
Western Blot (WB)
(Mouse Prr5 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Prr5 antibody detected the Prr5 protein (arrow).)
Related Product Information for anti-Prr5 antibody
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer (By similarity).
Product Categories/Family for anti-Prr5 antibody
References
Johnstone, C.N., et al. Genomics 85(3):338-351(2005). Shan, Z., et al. Gene 303, 55-61 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,523 Da
NCBI Official Full Name
proline-rich protein 5
NCBI Official Synonym Full Names
proline rich 5 (renal)
NCBI Official Symbol
Prr5
NCBI Official Synonym Symbols
C78947; Arhgap8; Protor1; AU043908; Protor-1; C030017C09Rik
NCBI Protein Information
proline-rich protein 5; protein observed with Rictor-1
UniProt Protein Name
Proline-rich protein 5
Protein Family
UniProt Gene Name
Prr5
UniProt Synonym Gene Names
Protor1; Protor-1
UniProt Entry Name
PRR5_MOUSE
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Product Notes
The Prr5 prr5 (Catalog #AAA6337791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prr5, CT (Prr5, Protor1, Proline-rich protein 5, Protein observed with Rictor-1) (Azide free) (HRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prr5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Prr5 prr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prr5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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