Ephb4 recombinant protein

Ephb4, 16-539aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206421

• Gene Names: Ephb4; Htk; MDK2; Myk1; Tyro11; AI042935; b2b2412Clo
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: Ephb4; 16-539aa; Mouse; His tag; Baculovirus; Ephrin type-B receptor 4 isoform b; Eph receptor B4; AI042935; Htk; MDK2; Myk1; Tyro11; EPHB4_HUMAN; Ephrin receptor; EphB4; Ephrin type-B receptor 4; Hepatoma Transmembrane Kinase; HTK; MYK 1; MYK1; TYRO 11; TYRO11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11; Ephb4 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: LEETLLNTKL ETADLKWVTY PQAEGQWEEL SGLDEEQHSV RTYEVCDMKR PGGQAHWLRT GWVPRRGAVH VYATIRFTMM ECLSLPRASR SCKETFTVFY YESEADTATA HTPAWMENPY IKVDTVAAEH LTRKRPGAEA TGKVNIKTLR LGPLSKAGFY LAFQDQGACM ALLSLHLFYK KCSWLITNLT YFPETVPREL VVPVAGSCVA NAVPTANPSP SLYCREDGQW AEQQVTGCSC APGYEAAESN KVCRACGQGT FKPQIGDESC LPCPANSHSN NIGSPVCLCR IGYYRARSDP RSSPCTTPPS APRSVVHHLN GSTLRLEWSA PLESGGREDL TYAVRCRECR PGGSCLPCGG DMTFDPGPRD LVEPWVAIRG LRPDVTYTFE VAALNGVSTL ATGPPPFEPV NVTTDREVPP AVSDIRVTRS SPSSLILSWA IPRAPSGAVL DYEVKYHEKG AEGPSSVRFL KTSENRAELR GLKRGASYLV QVRARSEAGY GPFGQEHHSQ TQLDESESWR EQLAVEHHHH HH
• Sequence Length: 987

• Applicable Applications for Ephb4 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ephb4 recombinant protein

Ephb4, also known as ephrin type-B receptor 4 isoform b, is a member of the Eph receptor tyrosine kinase family shown to act in neuronal guidance and mediate venal/arterial separation. Ephb4 provides survival advantage to squamous cell carcinoma of the head and neck. It has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Recombinant mouse Ephb4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ephb4 recombinant protein

Cardiovascular; Angiogenesis

References

Xiao Z. et al., (2012) Cancer Biol Ther. 13:630-637.; Masood R. et al., (2006) Int J Cancer. 119:1236-1248.; Dopeso H. et al., (2009) Cancer Res. 69:7430-7438.

NCBI and Uniprot Product Information

• NCBI GI #: 227330573
• NCBI GeneID: 13846
• NCBI Accession #: NP_034274
• NCBI GenBank Nucleotide #: NM_010144.6
• UniProt Accession #: P54761; Q60627; Q99MR2
• Molecular Weight: 58.7kDa (532aa) 50-70KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: ephrin type-B receptor 4 isoform b
• NCBI Official Synonym Full Names: Eph receptor B4
• NCBI Official Symbol: Ephb4
• NCBI Official Synonym Symbols: Htk; MDK2; Myk1; Tyro11; AI042935; b2b2412Clo
• NCBI Protein Information: ephrin type-B receptor 4
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb4
• UniProt Synonym Gene Names: Htk; Mdk2; Myk1; mDK-2

Uniprot Description

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.