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Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit PRPF8 Polyclonal Antibody | anti-PRPF8 antibody
PRPF8 Polyclonal Antibody
Gene Names
PRPF8; PRP8; RP13; HPRP8; PRPC8; SNRNP220
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purification
Synonyms
PRPF8; Polyclonal Antibody; PRPF8 Polyclonal Antibody; PRP8; RP13; HPRP8; PRPC8; SNRNP220; anti-PRPF8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
2335
Applicable Applications for anti-PRPF8 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:1000
IP: 1:20 - 1:50
IP: 1:20 - 1:50
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PRPF8
Calculated Molecular Weight
273kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-PRPF8 antibody
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
Product Categories/Family for anti-PRPF8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
2335
NCBI Official Full Name
Pre-mRNA-processing-splicing factor 8
NCBI Official Synonym Full Names
pre-mRNA processing factor 8
NCBI Official Symbol
PRPF8
NCBI Official Synonym Symbols
PRP8; RP13; HPRP8; PRPC8; SNRNP220
NCBI Protein Information
pre-mRNA-processing-splicing factor 8; p220; PRP8 homolog; splicing factor Prp8; apoptosis-regulated protein 1; apoptosis-regulated protein 2; 220 kDa U5 snRNP-specific protein; precursor mRNA processing protein; PRP8 pre-mRNA processing factor 8 homolog; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
UniProt Protein Name
Pre-mRNA-processing-splicing factor 8
Protein Family
UniProt Gene Name
PRPF8
UniProt Synonym Gene Names
PRPC8
UniProt Entry Name
PRP8_HUMAN
Similar Products
Product Notes
The PRPF8 prpf8 (Catalog #AAA9126231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRPF8 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRPF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500 - 1:1000 IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the PRPF8 prpf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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