Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF31 antibody (MBS9125786) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit PRPF31 Polyclonal Antibody | anti-PRPF31 antibody
PRPF31 Polyclonal Antibody
IHC: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF31 antibody (MBS9125786) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PRPF31 antibody (MBS9125786) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded rat heart using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded rat heart using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded human gastric cancer using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded human gastric cancer using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded mouse brain using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded mouse brain using PRPF31 antibody (MBS9125786) at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
Calculated MW: 30kDa/40kDa/54kDa/55kDa
NCBI Description
This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.[provided by RefSeq, Jan 2009]
Uniprot Description
PRPF31: Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation. Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant. Belongs to the PRP31 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Spliceosome; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: Cajal body; U4/U6 x U5 tri-snRNP complex; nuclear speck; nucleus
Molecular Function: ribonucleoprotein binding; protein binding
Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome
Disease: Retinitis Pigmentosa 11
Research Articles on PRPF31
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Product Notes
The PRPF31 prpf31 (Catalog #AAA9125786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRPF31 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRPF31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the PRPF31 prpf31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPF31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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