Rabbit anti-Human, Mouse PROX-1 Polyclonal Antibody | anti-PROX-1 antibody

PROX-1 (S514) (PROX1, Prospero Homeobox Protein 1, Homeobox Prospero-like Protein PROX1) (Biotin)

Cat. Num. AAA6337602

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PROX-1; Polyclonal Antibody; PROX-1 (S514) (PROX1; Prospero Homeobox Protein 1; Homeobox Prospero-like Protein PROX1) (Biotin); PROX1; Prospero homeobox protein 1; Homeobox prospero-like protein PROX1; anti-PROX-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PROX-1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PROX-1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 492-520 of human PROX-1. Swiss/UniProt Accession: Q92786
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS644835. PROX-1 (arrow) was detected usingMBS644835.)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) usingMBS644835. PROX-1 (arrow) was detected usingMBS644835.)

Testing Data

(All lanes: MBS6009082 (1:2000) Lane 1: HepG2 whole cell lysate (20ug/lane) Lane 2: SH-SY5Y whole cell lysate (20ug/lane) Lane 3: SW620 whole cell lysate (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS644835 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS644835 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PROX-1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

1. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 2. Zhang, C., et al. Clin. Chim. Acta 411 (5-6), 395-399 (2010). 3. Suresh, S., et al. FEBS J. 277(4):951-963(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 85702224
• NCBI GeneID: 5629
• UniProt Accession #: Q92786; Q5SW76; Q8TB91; A6NK29; A8K2B1
• Molecular Weight: 737
• NCBI Official Full Name: Prospero homeobox protein 1
• NCBI Official Synonym Full Names: prospero homeobox 1
• NCBI Official Symbol: PROX1
• NCBI Protein Information: prospero homeobox protein 1; prospero-related homeobox 1; homeobox prospero-like protein PROX1
• UniProt Protein Name: Prospero homeobox protein 1
• UniProt Gene Name: PROX1
• UniProt Synonym Gene Names: PROX-1
• UniProt Entry Name: PROX1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

PROX1: May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons. Belongs to the Prospero homeobox family.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor binding; protein binding; DBD domain binding; DNA binding; LBD domain binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; lens development in camera-type eye; circadian rhythm; neural tube development; positive regulation of cyclin-dependent protein kinase activity; ventricular cardiac myofibril development; muscle thin filament assembly; atrial cardiac muscle morphogenesis; positive regulation of transcription, DNA-dependent; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; olfactory placode formation; negative regulation of cell proliferation; venous blood vessel morphogenesis; pancreas development; positive regulation of cell proliferation; lymphangiogenesis; ventricular cardiac muscle morphogenesis; kidney development; response to nutrient levels; dentate gyrus development; negative regulation of transcription factor activity; positive regulation of cell cycle; liver development; regulation of circadian rhythm; cerebellar granule cell differentiation; dorsal spinal cord development; negative regulation of viral genome replication; regulation of gene expression; otic placode formation; embryonic retina morphogenesis in camera-type eye; positive regulation of endothelial cell proliferation; optic placode formation involved in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; retina morphogenesis in camera-type eye; lung development