Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Rabbit anti-Rat Proteinase 3 (PR3) Polyclonal Antibody | anti-PR3 antibody
Biotin-Linked Antibody to Proteinase 3 (PR3)
Reactivity
Rat
Applications
Immunohistochemistry, ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
Proteinase 3 (PR3); Polyclonal Antibody; Biotin-Linked Antibody to Proteinase 3 (PR3); anti-PR3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PR3. It has been selected for its ability to recognize PR3 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-ALV FGGAVQASKI VGGHEARPHS RPYVASLQLS RSPGSHFCGG TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ KFTITQVFEN NYNPEETLND VLLLQLNRPA SLGKQVAVAS LPQQDQSLSQ GTQCLAMGWG RLGTRAPTPR VLHELNVTVV TFLCREHNVC TLVPRRAAGI CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM YVNWIH
Sequence Length
254
Applicable Applications for anti-PR3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), ICC, IHC-P, IHC-F
Application Notes
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Product Categories/Family for anti-PR3 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
27,807 Da
NCBI Official Full Name
proteinase 3
UniProt Protein Name
Myeloblastin
UniProt Gene Name
PRTN3
UniProt Synonym Gene Names
MBN; PR-3; PR3; NP-4
UniProt Entry Name
PRTN3_HUMAN
Uniprot Description
PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.76; Protease; Cell cycle regulation
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol
Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: collagen catabolic process; negative regulation of phagocytosis; positive regulation of cell proliferation; proteolysis; blood coagulation
Similar Products
Product Notes
The PR3 prtn3 (Catalog #AAA2004748) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotin-Linked Antibody to Proteinase 3 (PR3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Proteinase 3 (PR3) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), ICC, IHC-P, IHC-F. Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PR3 prtn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-ALV FGGAVQASKI VGGHEARPHS RPYVASLQLS RSPGSHFCGG TLIHPRFVLT AAHCLQDISW QLVTVVLGAH DLLSSEPEQQ KFTITQVFEN NYNPEETLND VLLLQLNRPA SLGKQVAVAS LPQQDQSLSQ GTQCLAMGWG RLGTRAPTPR VLHELNVTVV TFLCREHNVC TLVPRRAAGI CFGDSGGPLI CNGILHGVDS FVIRECASLQ FPDFFARVSM YVNWIH. It is sometimes possible for the material contained within the vial of "Proteinase 3 (PR3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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