Rabbit Protein Serine/Threonine phosphatase 2A/B alpha Polyclonal Antibody | anti-PPP2R2A antibody

Protein Serine/Threonine phosphatase 2A/B alpha

Cat. Num. AAA618990

• Gene Names: PPP2R2A; B55A; PR52A; PR55A; B55ALPHA
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified; Purified by ammonium sulfate fractionation.
• Synonyms: Protein Serine/Threonine phosphatase 2A/B alpha; Polyclonal Antibody; Anti -Protein Serine/Threonine phosphatase 2A/B alpha; anti-PPP2R2A antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Protein Serine/Threonine phosphatase 2A/B alpha (55kD). Does not crossreact with other protein phosphatase isoforms. Species Crossreactivity: human, mouse, rat and bovine.
• Purity/Purification: Purified; Purified by ammonium sulfate fractionation.
• Form/Format: Supplied as a liquid in PBS, 0.08% sodium azide.

• Applicable Applications for anti-PPP2R2A antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 5-10ug/ml; Immunocytochemistry: 5-10ug/ml; Optimum dilutions to be determined by researcher.
• Immunogen: Synthetic peptide corresponding to aa14-27, NH2-Phe-Ser-Gln-Val- Lys-Gly-Ala-Val-Asp-Asp-Asp-Val-Ala-Glu-COOH, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PPP2R2A antibody

Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases.

Product Categories/Family for anti-PPP2R2A antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 119583982
• NCBI GeneID: 5520
• UniProt Accession #: P63151; P50409; Q00007; B2RBU8; B4E1T7
• Molecular Weight: 51,692 Da
• NCBI Official Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), alpha isoform, isoform CRA_b
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit B, alpha
• NCBI Official Symbol: PPP2R2A
• NCBI Official Synonym Symbols: B55A; PR52A; PR55A; B55ALPHA
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform; serine/threonine protein phosphatase 2A, 55 KDA regulatory subunit B, alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2R2A
• UniProt Entry Name: 2ABA_HUMAN

NCBI Description

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Uniprot Description

PPP2R2A: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Expressed in all tissues examined. Belongs to the phosphatase 2A regulatory subunit B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytosol

Molecular Function: protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; regulation of catalytic activity; mRNA catabolic process, nonsense-mediated decay; response to morphine; gene expression; mitotic cell cycle; signal transduction; protein amino acid dephosphorylation; G2/M transition of mitotic cell cycle