Rabbit Protein Serine/Threonine phosphatase 2A/A Polyclonal Antibody | anti-PPH22 antibody

Protein Serine/Threonine phosphatase 2A/A

Cat. Num. AAA611697

• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Purified; Purified
• Synonyms: Protein Serine/Threonine phosphatase 2A/A; Polyclonal Antibody; Anti -Protein Serine/Threonine phosphatase 2A/A; anti-PPH22 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PP 2A/A at 65kD. Species Crossreactivity: mouse, rat and bovine. Does not crossreact with other protein phosphatase isoforms.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.08% sodium azide.

• Applicable Applications for anti-PPH22 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunocytochemistry, and Immunoprecipitation.; Dilution: Western Blot: 5-10ug/ml; Immunocytochemistry: 10-15ug/ml; Immunoprecipitation: Can be used in conjunction with anti-PP2A/C to immunoprecipitate heterotrimeric PP2A; Optimum dilutions to be determined by researcher.
• Immunogen: Synthetic peptide corresponding to aa7-19, NH2-Asp-Asp-Ser-Leu-Tyr-Pro- Ile-Ala-Val-Leu-Ile-Asp-Glu-COOH, from the N-terminal of PP 2A/A regulatory subunit
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-PPH22 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 4210
• UniProt Accession #: P23595; D6VRG5
• Molecular Weight: 43,047 Da
• NCBI Official Full Name: protein serine /threonine phosphatase 2A
• UniProt Protein Name: Serine/threonine-protein phosphatase PP2A-2 catalytic subunit
• UniProt Gene Name: PPH22
• UniProt Synonym Gene Names: SIS4
• UniProt Entry Name: PP2A2_YEAST

Uniprot Description

Function: Exact function not known, phosphatase 2A performs an essential cellular function.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 1 manganese ion per subunit

By similarity.

Subunit structure: Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD2, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42. Ref.9 Ref.11

Post-translational modification: Reversibly methyl esterified on Leu-377 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Miscellaneous: Present with 4110 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the PPP phosphatase family. PP-2A subfamily.