Chicken Protein Kinase C, theta Polyclonal Antibody | anti-PRKCQ antibody

Protein Kinase C, theta (PKCt)

Cat. Num. AAA623729

• Gene Names: PRKCQ; PRKCT; MGC126514; MGC141919; nPKC-theta
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Protein Kinase C; theta; Polyclonal Antibody; theta (PKCt); Anti -Protein Kinase C; anti-PRKCQ antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Protein Kinase C, theta. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-PRKCQ antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide corresponding to aa610-673
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Test: E coli-derived fusion protein as test antigen. Affi-anti- PRKCQ IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-PRKCQ antibody

PKC theta is a calcium-independent, novel isoform that is important in transducing signals in T lymphocytes. Interaction of a T lymphocyte with an antigen-presenting cell results in clustering of the T cell antigen receptor (TCR) and the recruitment of a large signaling complex to the site of cell-cell contact. PKC theta is essential for TCR-mediated activation. PKC theta plays a role in pathways that link the TCR signaling complex to the activation of NF-kappa B in mature T lymphocytes.

Product Categories/Family for anti-PRKCQ antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 55957943
• NCBI GeneID: 5588
• UniProt Accession #: Q04759; Q14DH6; Q3MJF1; Q64FY5; Q9H508; Q9H549; Q5JUN8
• Molecular Weight: 81,865 Da
• NCBI Official Full Name: protein kinase C, theta
• NCBI Official Synonym Full Names: protein kinase C, theta
• NCBI Official Symbol: PRKCQ
• NCBI Official Synonym Symbols: PRKCT; MGC126514; MGC141919; nPKC-theta
• NCBI Protein Information: protein kinase C theta type; OTTHUMP00000019053
• UniProt Protein Name: Protein kinase C theta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCQ
• UniProt Synonym Gene Names: PRKCT
• UniProt Entry Name: KPCT_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq]

Uniprot Description

PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.

Protein type: Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; immunological synapse; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: positive regulation of interleukin-17 production; phototransduction, visible light; axon guidance; platelet activation; apoptosis; positive regulation of interleukin-2 biosynthetic process; membrane protein ectodomain proteolysis; protein ubiquitination; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; rhodopsin mediated signaling; positive regulation of interleukin-4 production; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; innate immune response; positive regulation of T cell proliferation; regulation of cell growth; positive regulation of T cell activation; blood coagulation; inflammatory response; cell structure disassembly during apoptosis