Rabbit Protein Kinase C, delta Polyclonal Antibody | anti-PRKCD antibody

Protein Kinase C, delta, phosphorylated (Ser645) (Protein Kinase C delta Type, nPKC-delta, PRKCD)

Cat. Num. AAA626758

• Gene Names: PRKCD; MAY1; PKCD; MGC49908; nPKC-delta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Protein Kinase C; delta; Polyclonal Antibody; phosphorylated (Ser645) (Protein Kinase C delta Type; nPKC-delta; PRKCD); Anti -Protein Kinase C; anti-PRKCD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Protein Kinase C, delta when phosphorylated at serine 645. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-PRKCD antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human PKCd around the phosphorylation site of serine 645 (R-L-SP-Y-S).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PRKCD antibody

Protein Kinase Cd (PKCd) is a 78kD member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKCd is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. In contrast to other PKC isozymes where phosphorylation of the activation loop residue is essential for catalytic activity, phosphorylation of the activation loop threonine 507 of PKCd appears to modulate, but is not required for, catalytic activity. This phosphorylation may also regulate PKCd stability and degradation. Autophosphorylation at serine 645 (serine 643 in mouse and rat) is not required for, but may modulate catalytic activity and receptor dephosphorylation.

Product Categories/Family for anti-PRKCD antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47157325
• NCBI GeneID: 5580
• NCBI Accession #: NP_997704.1
• NCBI GenBank Nucleotide #: NM_212539.1
• UniProt Accession #: Q05655; Q15144; Q86XJ6; B2R834
• Molecular Weight: 77,505 Da
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: PRKCD
• NCBI Official Synonym Symbols: MAY1; PKCD; MGC49908; nPKC-delta
• NCBI Protein Information: protein kinase C delta type; OTTHUMP00000165024; OTTHUMP00000165025; protein kinase C delta VIII
• UniProt Protein Name: Protein kinase C delta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCD
• UniProt Entry Name: KPCD_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Uniprot Description

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities.

Protein type: Kinase, protein; EC 2.7.11.13; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Protein kinase, AGC; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intercellular junction; nucleus; cytosol

Molecular Function: calcium-independent protein kinase C activity; protein serine/threonine kinase activity; protein binding; enzyme binding; protein kinase C activity; insulin receptor substrate binding; metal ion binding; enzyme activator activity; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: B cell proliferation; negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; interleukin-12 production; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of filopodium formation; positive regulation of superoxide release; regulation of receptor activity; negative regulation of protein binding; cell structure disassembly during apoptosis; epidermal growth factor receptor signaling pathway; platelet activation; negative regulation of peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; neutrophil activation; protein stabilization; negative regulation of actin filament polymerization; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; cell cycle; phospholipase C activation; negative regulation of inflammatory response; defense response to bacterium; interleukin-10 production; innate immune response; gene expression; immunoglobulin mediated immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; induction of apoptosis by oxidative stress

Disease: Autoimmune Lymphoproliferative Syndrome, Type Iii