Western Blot (WB)
(Western Blot Positive WB detected in: K562 whole cell lysateAll lanes: PADI2 antibody at 3ug/mlSecondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 50 kDaObserved band size: 76, 50 kDa)
Rabbit anti-Human Protein-arginine deiminase type-2 Polyclonal Antibody | anti-PADI2 antibody
Rabbit anti-human Protein-arginine deiminase type-2 polyclonal Antibody
Western Blot (WB)
(Western Blot Positive WB detected in: K562 whole cell lysateAll lanes: PADI2 antibody at 3ug/mlSecondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 50 kDaObserved band size: 76, 50 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: K562 whole cell lysateAll lanes: PADI2 antibody at 3ug/mlSecondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 76, 50 kDaObserved band size: 76, 50 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry image of MBS7000390 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0).Section was blocked with 10% normal goat serum 30min at RT.Then primary antibody (1% BSA) was incubated at 4°C overnight.The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunohistochemistry image of MBS7000390 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0).Section was blocked with 10% normal goat serum 30min at RT.Then primary antibody (1% BSA) was incubated at 4°C overnight.The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7000390 at 1:100, counter- stained with DAPI.The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum.The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7000390 at 1:100, counter- stained with DAPI.The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum.The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]
Uniprot Description
PADI2: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family.
Protein type: EC 3.5.3.15; Hydrolase
Chromosomal Location of Human Ortholog: 1p36.13
Cellular Component: cytoplasm
Molecular Function: calcium ion binding; estrogen receptor binding; protein-arginine deiminase activity
Biological Process: chromatin-mediated maintenance of transcription; estrogen receptor signaling pathway; peptidyl-citrulline biosynthetic process from peptidyl-arginine; substantia nigra development
Research Articles on PADI2
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Product Notes
The PADI2 padi2 (Catalog #AAA7000390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Protein-arginine deiminase type-2 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein-arginine deiminase type-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200. Researchers should empirically determine the suitability of the PADI2 padi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein-arginine deiminase type-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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