Rabbit anti-Human, Rat PROM1 Polyclonal Antibody | anti-PROM1 antibody

Anti-PROM1 antibody

Cat. Num. AAA176043

• Gene Names: PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: PROM1; Polyclonal Antibody; Anti-PROM1 antibody; Prominin-1; prominin 1; AC133 antibody; Antigen AC133 antibody; CD133 antibody; CORD12 antibody; hematopoietic stem cell antigen antibody; hProminin antibody; MCDR2 antibody; MSTP061 antibody; PROM1 antibody; PROM 1 antibody; PROM-1 antibody; Prominin-1 antibody; Prominin 1 antibody; Prominin1 antibody; Prominin-like protein 1 antibody; PROML1 antibody; RP41 antibody; STGD4 antibody; anti-PROM1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 865

• Applicable Applications for anti-PROM1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot; Concentration: 0.1-0.5 ug/ml; Tested Species: Hu, Rat; Predicted Species: -; Antigen Retrieval: -; ; Immunohistochemistry (Paraffin-embedded Section); Concentration: 0.5-1ug/ml; Tested Species: Hu; Predicted Species: -; Antigen Retrieval: By Heat; ; WB: The detection limit for PROM1 is approximately 0.5ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results. ; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested. ; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PROM1 (836-850aa MKNMENGNNGYHKDH), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C. After reconstitution, at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-PROM1 antibody, MBS176043, Western blottingLane 1: PANC Cell LysateLane 2: HELA Cell LysateLane 3: SW620 Cell LysateLane 4: COLO320 Cell LysateLane 5: A549 Cell Lysate )

Western Blot (WB)

(Figure 1. Western blot analysis of PROM1 using anti- PROM1 antibody (MBS176043).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: rat brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PROM1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PROM1 at approximately 130KD. The expected band size for PROM1 is at 97KD. )

Immunohistochemistry (IHC)

(Anti-PROM1 antibody, MBS176043, IHC(P)IHC(P): Human Tonsil Tissue )

Related Product Information for anti-PROM1 antibody

PROM1(Prominin 1), also called CD133, is a glycoprotein. Maw et al.(2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al.(2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al.(2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor.

NCBI and Uniprot Product Information

• NCBI GI #: 13124442
• NCBI GeneID: 8842
• UniProt Accession #: O43490; Q6SV49; Q6SV50; Q6SV51; Q6SV52; Q6SV53; Q96EN6
• Molecular Weight: 94,605 Da
• NCBI Official Full Name: Prominin-1
• NCBI Official Synonym Full Names: prominin 1
• NCBI Official Symbol: PROM1
• NCBI Official Synonym Symbols: RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
• NCBI Protein Information: prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen
• UniProt Protein Name: Prominin-1
• Protein Family: Prominin
• UniProt Gene Name: PROM1
• UniProt Synonym Gene Names: PROML1
• UniProt Entry Name: PROM1_HUMAN

NCBI Description

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: stereocilium; extracellular space; cell surface; photoreceptor outer segment; microvillus membrane; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; ER-Golgi intermediate compartment; plasma membrane; vesicle; brush border

Molecular Function: actinin binding; protein binding; cadherin binding

Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye

Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12