Rabbit anti-Human, Mouse Glypican 3 Polyclonal Antibody | anti-GPC3 antibody

Glypican 3, NT (Glypican-3, GPC3, DGSX, GTR2-2, Intestinal Protein OCI-5, MXR7, OCI5, OCI-5, SDYS, SGB, SGBS, SGBS1) (Azide free) (HRP)

Cat. Num. AAA6478587

• Gene Names: GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Glypican 3; Polyclonal Antibody; NT (Glypican-3; GPC3; DGSX; GTR2-2; Intestinal Protein OCI-5; MXR7; OCI5; OCI-5; SDYS; SGB; SGBS; SGBS1) (Azide free) (HRP); anti-GPC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPC3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GPC3 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 21-50 in the N-terminal region of human GPC3, UniProt Accession: #P51654.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HepG2 cell line lysates (35ug/lane) using G8235-50D. This demonstrates G8235-50D detected the GPC3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of mouse stomach tissue lysates (35ug/lane)using G8235-50D. This demonstrates the G8235-50D detected the GPC3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of G8235-50D in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-GPC3 antibody

Antibodies; Cancer Markers

References

1. Nakatsura, T., et al., Clin. Cancer Res. 10(19):6612-6621 (2004). 2. Boily, G., et al., Br. J. Cancer 90(8):1606-1611 (2004). 3. Wichert, A., et al., Oncogene 23(4):945-955 (2004). 4. Midorikawa, Y., et al., Int. J. Cancer 103(4):455-465 (2003). 5. Sung, Y.K., et al., Cancer Sci. 94(3):259-262 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4758462
• NCBI GeneID: 2719
• NCBI Accession #: NP_004475
• NCBI GenBank Nucleotide #: NM_004484.3
• UniProt Accession #: P51654; Q2L880; Q2L882; C9JLE3; G3V1R0
• Molecular Weight: 61.8kDa (544aa) 50-70kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: glypican-3 isoform 2
• NCBI Official Synonym Full Names: glypican 3
• NCBI Official Symbol: GPC3
• NCBI Official Synonym Symbols: SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• NCBI Protein Information: glypican-3
• UniProt Protein Name: Glypican-3
• Protein Family: Glypican
• UniProt Gene Name: GPC3
• UniProt Synonym Gene Names: OCI5
• UniProt Entry Name: GPC3_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; anchored to plasma membrane; Golgi lumen; integral to plasma membrane; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; protein binding

Biological Process: phototransduction, visible light; glycosaminoglycan metabolic process; negative regulation of peptidase activity; positive regulation of endocytosis; pathogenesis; osteoclast differentiation; embryonic hindlimb morphogenesis; bone mineralization; body morphogenesis; positive regulation of glucose import; glycosaminoglycan catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; negative regulation of epithelial cell proliferation; anatomical structure morphogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; anterior/posterior axis specification; lung development

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1