Rabbit anti-Human PROC Polyclonal Antibody | anti-PROC antibody

PROC, ID (PROC, Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, Vitamin K-dependent protein C light chain, Vitamin K-dependent protein C heavy chain, Activation peptide) (Biotin)

Cat. Num. AAA6337547

• Gene Names: PROC; PC; APC; PROC1; THPH3; THPH4
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PROC; Polyclonal Antibody; ID (PROC; Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; Vitamin K-dependent protein C light chain; Vitamin K-dependent protein C heavy chain; Activation peptide) (Biotin); Activation peptide; anti-PROC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PROC antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PROC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-206 amino acids from the Central region of human PROC.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PROC Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PROC antibody detected the PROC protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PROC Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(PROC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PROC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PROC antibody

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Product Categories/Family for anti-PROC antibody

Antibodies; Coagulation Factors

References

Tang, W., et al. Blood (2010). In press: Agapkina, Iu.V., et al. Mol. Biol. (Mosk.) 44(4):613-619(2010). Witt, I., et al. Blood Coagul. Fibrinolysis 5(4):651-653(1994) Zhang, L., et al. Blood 80(4):942-952(1992) Grundy, C.B., et al. Hum. Genet. 89(6):683-684(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4506115
• NCBI GeneID: 5624
• NCBI Accession #: NP_000303.1
• NCBI GenBank Nucleotide #: NM_000312.3
• UniProt Accession #: P04070; Q15189; Q15190; Q16001; Q53S74; Q9UC55
• Molecular Weight: 160,000
• NCBI Official Full Name: vitamin K-dependent protein C preproprotein
• NCBI Official Synonym Full Names: protein C (inactivator of coagulation factors Va and VIIIa)
• NCBI Official Symbol: PROC
• NCBI Official Synonym Symbols: PC; APC; PROC1; THPH3; THPH4
• NCBI Protein Information: vitamin K-dependent protein C; autoprothrombin IIA; anticoagulant protein C; blood coagulation factor XIV
• UniProt Protein Name: Vitamin K-dependent protein C
• Protein Family: Procalin
• UniProt Gene Name: PROC
• UniProt Entry Name: PROC_HUMAN

NCBI Description

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Uniprot Description

PROC: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal dominant (THPH3). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal recessive (THPH4). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare. Belongs to the peptidase S1 family.

Protein type: Protease; Apoptosis; EC 3.4.21.69

Chromosomal Location of Human Ortholog: 2q13-q14

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity; calcium ion binding

Biological Process: cellular protein metabolic process; negative regulation of blood coagulation; blood coagulation; proteolysis; post-translational protein modification; peptidyl-glutamic acid carboxylation; leukocyte migration; negative regulation of apoptosis

Disease: Thrombophilia Due To Protein C Deficiency, Autosomal Recessive; Thrombophilia Due To Protein C Deficiency, Autosomal Dominant